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Fetuin, a glycoprotein present in serum, is synthesized by hepatocytes and is an important inhibitor of soft tissue calcification. It is frequently used as a tissue culture medium supplement contributing to the attachment and spreading of cells. Fetuin is a member of the cystatin superfamily of proteins.

Target Species
Product Form
Purified bovine fetuin from fetal calf serum - lyophilized
Use sterile distilled water to give a solution of 1.0 mg/ml
Preservative Stabilisers
None present
Protein content: >80%
Total impurities: <0.3% Free N-acetyl-neuraminic acid
Protein Molecular Weight
48.4 kDa
For research purposes only
6 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Fetuin

  1. Dziegielewska, K. M. et al. (1990) The complete cDNA and amino acid sequence of bovine fetuin. Its homology with alpha 2HS glycoprotein and relation to other members of the cystatin superfamily.
    J. Biol. Chem. 265: 4354 - 4357.

Entrez Gene
GO Terms
GO:0006953 acute-phase response
GO:0005576 extracellular region
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0030502 negative regulation of bone mineralization
GO:0050727 regulation of inflammatory response
GO:0050766 positive regulation of phagocytosis


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