17-beta-Estradiol Antibody

17-beta-Estradiol Antibody gallery image 1

Published customer image:
Sheep anti 17-beta estradiol antibody used to assess circulating estradiol levels in the Hylid frog Smilisca sordida by radioimmune assay.
Image caption:
Circulating hormone levels in S. sordida.
Levels of progesterone (P), dihydrotestosterone (DHT), testosterone (T), estradiol (E2) and corticosterone (CORT) in (A) northern and southern latitude populations and (B) high and low elevations. Two asterisks indicate significance with a Benjamini and Hochberg correction and single asterisk indicates significance only when the correction was not applied. Bars represent ±1SE.

From: Leary CJ, Ralicki HF, Laurencio D, Crocker-Buta S, Malone JH (2018)
Assessing the links among environmental contaminants, endocrinology, and parasites to understand amphibian declines in montane regions of Costa Rica.
PLoS ONE 13(1): e0191183.

  • 17-beta-Estradiol Antibody thumbnail image 1
  • Sheep anti 17-beta-Estradiol
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7010-2650Edatasheet pdfdatasheet pdf0.25 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Sheep anti 17-beta-estradiol polyclonal antibody recognizes 17 β-estradiol, a mammalian estrogenic hormone. 17 β-estradiol is produced in the ovaries, placenta and testis and has a role in normal sexual development and the regulation of the menstrual cycle.

      The following cross reactivites of Sheep anti 17-beta-estradiol have been observed:

      17-beta Oestradiol 100%

      Aldosterone, Cortisol, Corticosterone, DHEA, Deoxycorticosterone, Pregnenolone, Progesterone, 17OH-P and Testosterone all <0.01%
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Anole lizardyes
      Horned lizardyes
      Agamid lizardyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Estradiol conjugated to bovine serum albumin
    • Purity
    • Approx. Protein Concentrations
      5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional 17-beta-Estradiol Antibody Formats

    Formats Applications Sizes available
    17-beta-Estradiol Antibody : Purified E 0.25 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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