TMB Core
TMB Core
- Product Type
- Accessory Reagent
- Specificity
- TMB Core
TMB CORE is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP). TMB CORE contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. TMB CORE produces a deep blue color during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm. |
- Product Form
- Ready to use TMB solution - liquid
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is photosensitive and should be protected from light.
Avoid exposure to heat and contamination with metal ions or peroxidase.
Store in bottles made of High Density Polyethylene (HDPE).
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | Neat |
- Instructions For Use
-
BUF056B
1. Pour the desired amount of BUF056B into a sealable container and allow it to reach room temperature in the dark. Note that 0.1ml will be required per well.
2. Add 0.1ml of BUF056B per microtiter well.
3. Allow development of BUF056B. Development time is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation. i.e. wrapped in tinfoil.
4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.
5. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF056B is not recommended). -
BUF056A
1. Pour the desired amount of BUF056A into a sealable container and allow it to reach room temperature in the dark. Note that 0.1ml will be required per well.
2. Add 0.1ml of BUF056A per microtiter well.
3. Allow development of BUF056A. Development time is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation. i.e. wrapped in tinfoil.
4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.
5. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF056A is not recommended).
References for TMB Core
-
McKeand, S.A. et al. (2023) Inhibition of Neisseria gonorrhoeae complement-mediated killing during acute gonorrhoea is dependent upon the IgG2:IgG3 antibody ratio
bioRχiv . Sept 26 [Preprint-not reviewed].
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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