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TBS/Tween

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20x Tris Buffered Saline with Tween 20

Product Type
Accessory Reagent
Specificity
TBS/Tween

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BUF028
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20x Tris Buffered Saline with Tween 20 thumbnail image 1 20x Tris Buffered Saline with Tween 20 thumbnail image 2 20x Tris Buffered Saline with Tween 20 thumbnail image 3
20x Tris Buffered Saline with Tween 20 gallery image 1
Published customer image:
TBS/Tween (BUF028) used as a washing reagent for western blot membranes.
Image caption:
ADAMTS1 is up-regulated in non-small cell lung cancer tumor tissues.
(B) Western blot was used for evaluating the protein expression of ADAMTS1 in NSCLC tumor tissues and adjacent normal tissues with different clinical grades (n=12).

From: Hu X, Jiang C, Hu N, Hong S.
ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-β.
Aging (Albany NY). 2023 Mar 20;15(6):2097-2114.
doi: 10.18632/aging.204594.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
20x Tris Buffered Saline with Tween 20 gallery image 2
Published customer image:
TBS/Tween (BUF028) used as a washing reagent for western blotting membranes.
Image caption:
USP22 enhances YAP protein stability. (A) A375 cells were transfected different doses of USP22 construct for 48 h before being subjected to western blot for analysis of YAP expression. A375 cells were infected with USP22 shRNA virus and selected with puromycin for 72 h. Cells were harvested and subjected to (B) western blot and (C) reverse transcription-quantitative PCR for analysis of YAP protein and mRNA levels, respectively. (D) Half-life of YAP was examined using cycloheximide-chase assays. n.s., non-significant vs. shGFP. YAP, yes-associated protein; USP22, ubiquitin-specific peptidase 22; sh, short hairpin; CHX, cycloheximide; IB, immunoblot.

From: Wei Y, Jiang Z, Lu J.
USP22 promotes melanoma and BRAF inhibitor resistance via YAP stabilization.
Oncol Lett. 2021 May;21(5):394.
doi: 10.3892/ol.2021.12655.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
20x Tris Buffered Saline with Tween 20 gallery image 3
Published customer image:
Tris Buffered Saline with Tween 20 (BUF028) used as a washing reagent for protein measurement by western blotting.
Image caption:
Cocaine and morphine induce the expression of functional ZFP189 transcription factor in vitro. (C) ZFP189 protein expression in N2a cells in response to exposure to cocaine at multiple doses. Increasing the dosage of applied cocaine induced significant increases in ZFP189 protein expression. Ordinary one-way ANOVA relative to 0 μM condition (Dunnett Test); *p-value <0.05, ****p-value <0.0001, n = 4-8 wells per condition. (D) ZFP189 protein expression in N2a cells in response to exposure to morphine at multiple doses. Increasing the dosage of applied morphine induced significant increases in ZFP189 protein expression. Ordinary one-way ANOVA relative to 0 μM condition (Dunnett Test); *p-value <0.05, ****p-value <0.0001, n = 9 wells per condition.

From: Picone JA, Hassan A, Kim RK, Lira DP, Silva GM, Truby NL, Johnson HY, Teague CD, Neve RL, Banks ML, Cui X, Hamilton PJ.
The function of the ZFP189 transcription factor in the nucleus accumbens facilitates cocaine-specific transcriptional and behavioral adaptations.
Mol Psychiatry. 2024 30(6):2490-2503..
doi: 10.1038/s41380-024-02852-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Intended Use
Add 100ml TBS solution to 1.9 litres distilled water to prepare solution of TBS/0.1% Tween 20 for use in washing of sections during immunohistology staining procedures.
Product Form
Concentrated liquid
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature.
Store stock solution at room temperature.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for TBS/Tween

  1. Hu, X. et al. (2023) ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-β.
    Aging (Albany NY). 15 (6): 2097-2114.
  2. Ciupińska-Kajor, M. et al. (2013) Hepatic angiogenesis and fibrosis are common features in morbidly obese patients.
    Hepatol Int. 7 (1): 233-40.
  3. Wei, Y. et al. (2021) USP22 promotes melanoma and BRAF inhibitor resistance via YAP stabilization.
    Oncol Lett. 21 (5): 394.
  4. Zhaliazka, K. & Kurouski, D. (2023) Nanoscale imaging of individual amyloid aggregates extracted from brains of Alzheimer and Parkinson patients reveals presence of lipids in α-synuclein but not in amyloid β(1-42) fibrils.
    Protein Sci. 32 (4): e4598.
  5. Gau, D.M. & Roy, P. (2020) An in vitro Assay to Screen for Substrates of PKA Using Phospho-motif-specific Antibodies.
    Bio Protoc. 10 (8) [Epub ahead of print].
  6. Picone, J.A. et al. (2024) The function of the ZFP189 transcription factor in the nucleus accumbens facilitates cocaine-specific transcriptional and behavioral adaptations.
    Mol Psychiatry. Nov 25 [Epub ahead of print].

Western Blotting

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"