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Streptavidin:Alk. Phos.

Product Type
Accessory Reagent

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SDS Safety Datasheet SDS
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Alkaline phosphatase conjugated streptavidin is a ~53 kDa protein with a high binding affinity for biotin showing negligable binding to non biotinylated proteins.

Product Form
Streptavidin conjugated to alkaline phosphatase - liquid
Buffer Solution
0.05M TRIS Chloride
0.15M NaCl
0.001M MgCl2
0.0001M ZnCl2
50% (v/v) Glycerol; pH8.0
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
For research purposes only
12 months from date of despatch

Store at +4oC.


This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/10,000
Immunohistology - Frozen 1/50 1/1000
Immunohistology - Paraffin 1/50 1/1000
Western Blotting 1/1000 1/4000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Streptavidin

  1. Wright, A.K. et al. (2012) Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
    PLoS Pathog. 8: e1002622.
  2. Ahmed, U.K. et al. (2016) The Carbohydrate-linked Phosphorylcholine of the Parasitic Nematode Product ES-62 Modulates Complement Activation.
    J Biol Chem. 291 (22): 11939-53.
  3. Pennington, S.H. et al. (2018) Longevity of duodenal and peripheral T-cell and humoral responses to live-attenuated Salmonella typhi. strain Ty21a.
    Vaccine. 36 (31): 4725-33.


153090 159374

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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