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Streptavidin

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Streptavidin conjugated to HRP thumbnail image 1 Streptavidin conjugated to HRP thumbnail image 2 Streptavidin conjugated to HRP thumbnail image 3
Streptavidin conjugated to HRP gallery image 1
Detection of Rabbit anti Mouse TNFα:biotin in a sandwich ELISA with recombinant murine TNFα (PMP24) as the antigen and Rat anti Mouse TNFα (MCA1487) as the coating antibody using Streptavidin:HRP (STAR5B)
Streptavidin conjugated to HRP gallery image 2
Detection of Mouse anti histidine tag:biotin (MCA1396B) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (STAR5B)
Streptavidin conjugated to HRP gallery image 3
A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in BUF030A. After washing with BUF031A and blocking with BUF032A samples were detected using MCA1783B antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate STAR5B at 1/1000. Finally, BUF056A was used as substrate. Data is presented as the mean of three measurements.

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E WB

Streptavidin:HRP

Product Type
Accessory Reagent
Specificity
Streptavidin

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Product Code Applications Pack Size List Price Your Price Qty
STAR5B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P WB 1 mg
List Price Your Price

Streptavidin: horseradish peroxidase conjugates show negligible non-specific binding to non-biotinylated macromolecules and therefore give very low backgrounds.

Product Form
Purified Streptavidin conjugated to Horseradish Peroxidase (HRP) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Approx. Protein Concentrations
Protein concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Immunohistology - Frozen 1/500
Immunohistology - Paraffin 1/500
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for Streptavidin

  1. Stager, S. et al. (2000) Immunization with a recombinant stage-regulated surface protein from Leishmania donovani induces protection against visceral Leishmaniasis.
    J. Immunol. 165: 7064-7071.
  2. Weiss, D. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J. Leukoc. Biol. 83: 48-55.
  3. Wong, Y.L. et al. (2016) Adverse effects of hydroxyethyl starch (HES 130/0.4) on intestinal barrier integrity and metabolic function are abrogated by supplementation with Albumin.
    J Transl Med. 14: 60.
  4. Kuo, S.H. et al. (2017) Cerebellar Pathology in Early Onset and Late Onset Essential Tremor.
    Cerebellum. 16 (2): 473-482.
  5. Pokrovsky, V.S. et al. (2016) Comparative immunogenicity and structural analysis of epitopes of different bacterial L-asparaginases.
    BMC Cancer. 16: 89.

STAR5B

153043 158511 158528 160922 161553 161652 165031

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