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Streptavidin

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Streptavidin conjugated to HRP thumbnail image 1 Streptavidin conjugated to HRP thumbnail image 2 Streptavidin conjugated to HRP thumbnail image 3 Streptavidin conjugated to HRP thumbnail image 4
Streptavidin conjugated to HRP gallery image 1
Detection of Rabbit anti Mouse TNFα:biotin in a sandwich ELISA with recombinant murine TNFα (PMP24) as the antigen and Rat anti Mouse TNFα (MCA1487) as the coating antibody using Streptavidin:HRP (STAR5B)
Streptavidin conjugated to HRP gallery image 2
Detection of Mouse anti histidine tag:biotin (MCA1396B) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (STAR5B)
Streptavidin conjugated to HRP gallery image 3
A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in BUF030A. After washing with BUF031A and blocking with BUF032A samples were detected using MCA1783B antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate STAR5B at 1/1000. Finally, BUF056A was used as substrate. Data is presented as the mean of three measurements.
Streptavidin conjugated to HRP gallery image 4
Published customer image:
HRP conjugated Sttreptavidin used as a tertiary reagent to detect bound Biotinylated Goat anti Mouse IgG1 antibody (107008) used to detect bound Mouse anti Human synaptophysin antibody, clone SY38 (8479-0004) to identify synaptophysin in tongues of developinng fetus's by immunohistochemistry.
Image caption:
Tongue epithelium development:
3 (B) Age 20 weeks: Faint expression of Synaptophysin recorded (10x). 4 (C) Age 22 weeks: Faint expression of Synaptophysin is seen in the papilla (40x).

From:Sen, S., Mishra, S. & Kaul, J. M. (2014).
Study of Maturation of Synapses in the Human Tongue Epithelium Using Synaptophysin as Marker: An Immunohistochemical Approach.
Journal of Pioneering Medical Sciences, 4(3), 105-111.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Streptavidin:HRP

Product Type
Accessory Reagent
Specificity
Streptavidin

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Product Code Applications Pack Size List Price Your Price Qty
STAR5B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P WB 1 mg loader
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Streptavidin: horseradish peroxidase conjugates show negligible non-specific binding to non-biotinylated macromolecules and therefore give very low backgrounds.

Product Form
Purified Streptavidin conjugated to Horseradish Peroxidase (HRP) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Approx. Protein Concentrations
Protein concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Immunohistology - Frozen 1/500
Immunohistology - Paraffin 1/500
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for Streptavidin

  1. Stäger, S. et al. (2000) Immunization with a recombinant stage-regulated surface protein from Leishmania donovani induces protection against visceral leishmaniasis.
    J Immunol. 165 (12): 7064-71.
  2. Weiss, D. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J. Leukoc. Biol. 83: 48-55.
  3. Sen, S. et al. (2014) Study of Maturation of Synapses in the Human Tongue Epithelium Using Synaptophysin as Marker: An Immunohistochemical Approach.
    Journal of Pioneering Medical Sciences, 4(3), 105-111.
  4. Wong, Y.L. et al. (2016) Adverse effects of hydroxyethyl starch (HES 130/0.4) on intestinal barrier integrity and metabolic function are abrogated by supplementation with Albumin.
    J Transl Med. 14: 60.
  5. Pokrovsky, V.S. et al. (2016) Comparative immunogenicity and structural analysis of epitopes of different bacterial L-asparaginases.
    BMC Cancer. 16: 89.
  6. Kuo, S.H. et al. (2017) Cerebellar Pathology in Early Onset and Late Onset Essential Tremor.
    Cerebellum. 16 (2): 473-482.
  7. Kushwaha, V. & Murthy, P. (2021) Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents
    Asian Pacific Journal of Tropical Medicine. 14 (7): 287.
  8. Bouroutzika, E. et al. (2023) Melatonin Administration to Pregnant Ewes for Coccidiosis Control in Their Offspring.
    Animals (Basel). 13 (14): 2381.
    9. View The Latest Product References
  9. Kushwaha, V. & Capalash, N. (2023) Evaluation of immunomodulatory potential of recombinant histidyl-tRNA synthetase (rLdHisRS) protein of Leishmania donovani as a vaccine candidate against visceral leishmaniasis.
    Acta Trop. 241: 106867.

STAR5B

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