Streptavidin

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/100	1/200
Immunohistology - Frozen		1/100	1/500
Immunohistology - Paraffin		1/100	1/500

Flow Cytometry

Use 50ul of the suggested working dilution to label 10⁶ cells in 100ul.

References for Streptavidin

1. Raeber, A. et al. (1999) Ectopic expression of prion protein (PrP) in T lymphocytes or hepatocytes of PrP knockout mice is insufficient to sustain prion replication.
   Proc. Natl. Acad. Sci. USA. 96: 3987 - 3992.
2. Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.
   Vet Microbiol. 131:237-46.
3. Tsai, C.M. et al (2011) Galectin-1 and galectin-8 have redundant roles in promoting plasma cell formation.
   J Immunol. 187: 1643-52.
4. Ávila, L.R. et al. (2018) Promastigote parasites cultured from the lesions of patients with mucosal leishmaniasis are more resistant to oxidative stress than promastigotes from a cutaneous lesion.
   Free Radic Biol Med. 129: 35-45.

Immunofluorescence