Streptavidin
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Product Details
- Product Form
- Purified streptavidin from Streptomyces avidinii - lyophilised
- Reconstitution
- Reconstitute with distilled water or saline.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified streptavidin prepared by affinity chromatography
- Buffer Solution
- Phosphate/Borate buffered saline
- Preservative Stabilisers
- 1% Mannitol
1% Dextran - Activity
- 17 U/mg streptavidin
One unit will bind 1 µg of D-biotin. - Purity
- SDS PAGE: >95%
- Molecular Weight
- 53 kDa
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- Please see label for expiry date.
More Information
- Regulatory
- For research purposes only
Applications of Streptavidin
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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