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Streptavidin

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8429-9004
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SDS Safety Datasheet SDS
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Product Form
Purified streptavidin from Streptomyces avidinii - lyophilized
Reconstitution
Reconstitute with distilled water or saline.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified streptavidin prepared by affinity chromatography
Buffer Solution
0.01M Sodium Phosphate
0.01M Sodium Borate
0.15M Sodium Chloride
Preservative Stabilisers
1% Mannitol
1% Dextran
Activity
17 U/mg streptavidin
One unit will bind 1 µg of D-biotin.
Purity
>95% by SDS-PAGE
Protein Molecular Weight
53 kDa
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

8429-9004

153661 155319 157172 157410 158255 161281 163429 166684 167721 169437

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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