Red Cell Lysing Buffer

The 10X concentrated ERYTHROLYSE should be stored at room temperature and is stable for 1 year. Do not use if any discolouration or precipitation occurs in the ERYTHROLYSE solution. The information regarding the products is believed to be accurate, but the information and products have no warranty or guarantee other than those specified, since the ultimate conditions of use and the variability of the materials are beyond our control. We cannot be responsible for and we do not intend these products or the accompanying information to be used for patent infringement or other such violations.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry

Instructions For Use

Bio-Rad ERYTHROLYSE is supplied as a 10X concentration solution which must be diluted 1/10 with distilled water before use.

Bio-Rad ERYTHROLYSE is designed for use in whole blood immunofluorescent staining procedures and is suitable for use with human, rat and mouse blood.

Following staining according to standard procedures (worksheets available on request), 2ml of diluted ERYTHROLYSE is added to 100ul of whole blood, mixed well and incubated for 10 minutes at room temperature. The tubes should then be centrifuged (400g, 5 minutes), the supernatant decanted and cells washed once in 2ml PBS/1% BSA before analysis by flow cytometry.

Bio-Rad ERYTHROLYSE is supplied as a 10X concentration solution which must be diluted 1/10 with distilled water before use.

Bio-Rad ERYTHROLYSE is designed for use in whole blood immunofluorescent staining procedures and is suitable for use with human, rat and mouse blood.

Following staining according to standard procedures (worksheets available on request), 2ml of diluted ERYTHROLYSE is added to 100ul of whole blood, mixed well and incubated for 10 minutes at room temperature. The tubes should then be centrifuged (400g, 5 minutes), the supernatant decanted and cells washed once in 2ml PBS/1% BSA before analysis by flow cytometry.

References for Red Cell Lysing Buffer

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