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Quantum™ Simply Cellular® IgG

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Quantum™ Simply Cellular® Mouse IgG

Product Type
Accessory Reagent
Specificity
Quantum™ Simply Cellular® IgG

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Product Code Applications Pack Size List Price Your Price Qty
FCSC815C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 280 Tests loader
List Price Your Price
loader
FCSC815B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader
FCSC815A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 20 Tests loader
List Price Your Price
loader

Quantum™ Simply Cellular® Mouse IgG is comprised of four coated populations and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9μm). Each of the four sets is coated with goat-anti-mouse IgG (Fc-specific) with different calibrated Antibody Binding Capacities (ABC) for mouse monoclonal antibodies. The blank population has no specific binding capacity for mouse IgG.

When the bead populations are labeled in the same manner as the cells to be analyzed, they provide a means for constructing a QuickCal® calibration curve (ABC values vs. fluorescence intensity), from which samples may be "read". Cellular antigen expression may thus be quantitated in ABC units. The calibration curve is also a useful means for determining instrument linearity.

Intended Use
FCSC815C: FCSC815C is suitable for the quantitation of cellular antigen expression in Antibody Binding Capacity (ABC) units. This product may also serve as an accurate compensation standard when labeled with the same antibodies used to stain cell samples.
FCSC815B: FCSC815B is suitable for the quantitation of cellular antigen expression in Antibody Binding Capacity (ABC) units. This product may also serve as an accurate compensation standard when labeled with the same antibodies used to stain cell samples.
FCSC815A: FCSC815A is suitable for the quantitation of cellular antigen expression in Antibody Binding Capacity (ABC) units. This product may also serve as an accurate compensation standard when labeled with the same antibodies used to stain cell samples.
Buffer Solution
FCSC815C, FCSC815B: Borate buffered saline.
FCSC815A: Borate buffer.
Preservative Stabilisers
FCSC815C, FCSC815B: 0.1% Bovine Serum Albumin
0.05% Tween 20
10mM EDTA
0.09% Sodium Azide (NaN3)
FCSC815A: 0.1% Bovine Serum Albumin
0.05% Tween 20
10mM EDTA
0.09% Sodium Azide (NaN3).
Reagents In The Kit
FCSC815C: 2 x 7ml bottles of uncoated microbeads
8 x 7ml bottles of coated microbeads.
FCSC815B: 1 x 5ml bottle of uncoated microbeads
4 x 5ml bottles of coated microbeads.
FCSC815A: 1 x 1ml bottle of uncoated microbeads
4 x 1ml bottles of coated microbeads.
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
Quantum and Simply Cellular are trademarks of Bangs Laboratories, INC.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
FCSC815C
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC815C.pdf
FCSC815B
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC815B.pdf
FCSC815A
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC815A.pdf

References for Quantum™ Simply Cellular® IgG

  1. Park, J.A. & Cheung, N.V. (2022) Overcoming tumor heterogeneity by ex vivo. arming of T cells using multiple bispecific antibodies.
    J Immunother Cancer. 10 (1) :e003771.
  2. Bryniarski, M.A. et al. (2024) Utility of Cellular Measurements of Non-Specific Endocytosis to Assess the Target-Independent Clearance of Monoclonal Antibodies
    bioRχiv 16 Apr [Preprint].

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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