Quantum™ Simply Cellular® IgG
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Quantum™ Simply Cellular® Mouse IgG is comprised of four coated populations and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9μm). Each of the four sets is coated with goat-anti-mouse IgG (Fc-specific) with different calibrated Antibody Binding Capacities (ABC) for mouse monoclonal antibodies. The blank population has no specific binding capacity for mouse IgG.
When the bead populations are labeled in the same manner as the cells to be analyzed, they provide a means for constructing a QuickCal® calibration curve (ABC values vs. fluorescence intensity), from which samples may be "read". Cellular antigen expression may thus be quantitated in ABC units. The calibration curve is also a useful means for determining instrument linearity.
When the bead populations are labeled in the same manner as the cells to be analyzed, they provide a means for constructing a QuickCal® calibration curve (ABC values vs. fluorescence intensity), from which samples may be "read". Cellular antigen expression may thus be quantitated in ABC units. The calibration curve is also a useful means for determining instrument linearity.
Product Details
- Intended Use
- FCSC815A is suitable for the quantitation of cellular antigen expression in Antibody Binding Capacity (ABC) units. This product may also serve as an accurate compensation standard when labeled with the same antibodies used to stain cell samples.
- Buffer Solution
- Pack Size: 20 TestsBorate bufferPack Size: 100 Tests, 280 TestsBorate buffered saline
- Preservative Stabilisers
- 0.1% Bovine Serum Albumin
0.05% Tween 20
10mM EDTA
0.09% Sodium Azide (NaN3) - Reagents in the Kit
- Pack Size: 20 Tests1 x 1ml bottle of uncoated microbeads
4 x 1ml bottles of coated microbeadsPack Size: 100 Tests1 x 5ml bottle of uncoated microbeads
4 x 5ml bottles of coated microbeadsPack Size: 280 Tests2 x 7ml bottles of uncoated microbeads
8 x 7ml bottles of coated microbeads
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Acknowledgements
- Quantum™ and Simply Cellular® are trademarks of Bangs Laboratories, INC.
- Regulatory
- For research purposes only
Applications of Quantum™ Simply Cellular® IgG
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC815A.pdf
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