Quantum™ Simply Cellular® IgG

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Quantum™ Simply Cellular® Mouse IgG

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
FCSC815A F 20 Tests
FCSC815B F 100 Tests
FCSC815C F 280 Tests
FCSC815A is comprised of four coated populations and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9μm). Each of the four sets is coated with goat-anti-mouse IgG (Fc-specific) with different calibrated Antibody Binding Capacities (ABC) for mouse monoclonal antibodies. The blank population has no specific binding capacity for mouse IgG.

When the bead populations are labeled in the same manner as the cells to be analyzed, they provide a means for constructing a QuickCal® calibration curve (ABC values vs. fluorescence intensity), from which samples may be "read". Cellular antigen expression may thus be quantitated in ABC units. The calibration curve is also a useful means for determining instrument linearity.

Product Details

Intended Use
FCSC815A is suitable for the quantitation of cellular antigen expression in Antibody Binding Capacity (ABC) units. This product may also serve as an accurate compensation standard when labeled with the same antibodies used to stain cell samples.
Buffer Solution
Pack Size: 20 Tests
Borate buffer
Pack Size: 100 Tests, 280 Tests
Borate buffered saline
Preservative Stabilisers
0.1% Bovine Serum Albumin
0.05% Tween 20
10mM EDTA
0.09% Sodium Azide (NaN3)
Reagents in the Kit
Pack Size: 20 Tests
1 x 1ml bottle of uncoated microbeads
4 x 1ml bottles of coated microbeads
Pack Size: 100 Tests
1 x 5ml bottle of uncoated microbeads
4 x 5ml bottles of coated microbeads
Pack Size: 280 Tests
2 x 7ml bottles of uncoated microbeads
8 x 7ml bottles of coated microbeads

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
Please see label for expiry date

More Information

Acknowledgements
Quantum™ and Simply Cellular® are trademarks of Bangs Laboratories, INC.
Regulatory
For research purposes only

Applications of Quantum™ Simply Cellular® IgG

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC815A.pdf