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HRP-STABILIZING Diluent

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AbGUARD® HRP Stabilizer Plus

Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
BUF052A
Datasheet
C E P WB
SDS
100 ml

BUF052B
Datasheet
C E P WB
SDS
500 ml

BUF052C
Datasheet
C E P WB
SDS
1000 ml

Product Details

Intended Use
AbGuard® HRP Stabilizer Plus can be used for the direct dilution and long-term stabilization/storage of Horseradish Peroxidase (HRP)-conjugated proteins and antibodies at both low and high protein concentrations.

HRP-conjugated proteins diluted with AbGuard® HRP Stabilizer Plus can be stored at 4oC for a minimum of 30 months, and can withstand temperatures up to 37oC for 2 weeks or more.

AbGuard® HRP Stabilizer Plus stabilizes the HRP enzyme and optimizes the signal-to-noise ratio in most assays. Bio-Rad recommends the use of our TMB range for this purpose.

Please note - this product cannot be used for the dilution of anti-rabbit immunoglobulin antibodies e.g Goat anti-Rabbit Ig etc.
Product Form
Ready to use - liquid
Preparation
Weakly white liquid based on a Tris buffer with proprietary stabilizing components. Does not contain azide, mercury, BSA or other bovine material. Does contain weak concentrations of rabbit antibodies.
Preservative Stabilisers
<15ppm CMIT/MIT

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Regulatory
For research purposes only

Applications of HRP-STABILIZING Diluent

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for HRP-STABILIZING Diluent

  1. Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
    Biol Trace Elem Res. 164 (2): 205-11.
  2. Gholami, M.R. et al. (2020) Protective effects of honey, Apis mellifera meda. skorikov, on ischemia-reperfusion induced muscle injury.
    Int J Morphol., 38 (3): 804-10,

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