Flow Cytometry Absolute Count Standard™

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Flow Cytometry Absolute Count Standard™

Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
20 Tests

Flow Cytometry Absolute Count Standard™ is a suspension of microspheres that are approximately the size of human lymphocytes (7-9μm) and are presented as a known concentration. They are internally labeled with multiple fluorochromes and the combination of dyes allows the beads to be excited by a common argon laser (488nm) and emit in the three standard channels of a flow cytometer (FL1, FL2, FL3).

Product Details

Intended Use
Flow Cytometry Absolute Count Standard™ is intended for use as an internal counting standard. It is designed for use in the proper set-up of flow cytometers and cell counters and for the accurate enumeration of cells or particles.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Gelatin
≤ 0.05% Tween 20
0.09% Sodium Azide (NaN3)
Reagents in the Kit
1 bottle containing 10ml of microspheres

Storage Information

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guaranteed until date of expiry. Please see product label.

More Information

Absolute Count Standard™ is a trademark of Bangs Laboratories, INC.
For research purposes only

Applications of Flow Cytometry Absolute Count Standard™

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC580.pdf

Product Specific References

References for Flow Cytometry Absolute Count Standard™

  1. McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
    FASEB J. 25 (6): 1972-82.
  2. Dunsterville, C. et al. (2019) The Use of Dual-Cell-Tracker Dye Staining for the Identification and Characterization of Peanut-Specific T-Cell Subsets.
    Methods Mol Biol. 2020: 143-52.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra