Flow Cytometry Absolute Count Standard™
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Flow Cytometry Absolute Count Standard™ is a suspension of microspheres that are approximately the size of human lymphocytes (7-9μm) and are presented as a known concentration. They are internally labeled with multiple fluorochromes and the combination of dyes allows the beads to be excited by a common argon laser (488nm) and emit in the three standard channels of a flow cytometer (FL1, FL2, FL3).
Product Details
- Intended Use
- Flow Cytometry Absolute Count Standard™ is intended for use as an internal counting standard. It is designed for use in the proper set-up of flow cytometers and cell counters and for the accurate enumeration of cells or particles.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Gelatin
≤ 0.05% Tween 20
0.09% Sodium Azide (NaN3) - Reagents in the Kit
- 1 bottle containing 10ml of microspheres
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Acknowledgements
- Absolute Count Standard™ is a trademark of Bangs Laboratories, INC.
- Regulatory
- For research purposes only
Applications of Flow Cytometry Absolute Count Standard™
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC580.pdf
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Flow Cytometry Absolute Count Standard™
-
McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
FASEB J. 25 (6): 1972-82. -
Dunsterville, C. et al. (2019) The Use of Dual-Cell-Tracker Dye Staining for the Identification and Characterization of Peanut-Specific T-Cell Subsets.
Methods Mol Biol. 2020: 143-52.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
