Flow Cytometry Absolute Count Standard™

Flow Cytometry Absolute Count Standard™

Product Type
Accessory Reagent
Specificity
Flow Cytometry Absolute Count Standard™

Product Code Applications Pack Size List Price Your Price Qty
FCSC580
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 20 Tests
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Flow Cytometry Absolute Count Standard is a suspension of microspheres that are approximately the size of human lymphocytes (7-9μm) and are presented as a known concentration. They are internally labeled with multiple fluorochromes and the combination of dyes allows the beads to be excited by a common argon laser (488nm) and emit in the three standard channels of a flow cytometer (FL1, FL2, FL3).

Intended Use
Flow Cytometry Absolute Count Standard is intended for use as an internal counting standard. It is designed for use in the proper set-up of flow cytometers and cell counters and for the accurate enumeration of cells or particles.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Gelatin
≤ 0.05% Tween 20
0.09% Sodium Azide (NaN3)
Reagents in the Kit
1 bottle containing 10ml of microspheres.
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
Absolute Count Standard is a trademark of Bangs Laboratories, INC.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC580.pdf

References for Flow Cytometry Absolute Count Standard™

  1. McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
    FASEB J. 25 (6): 1972-82.
  2. Dunsterville, C. et al. (2019) The Use of Dual-Cell-Tracker Dye Staining for the Identification and Characterization of Peanut-Specific T-Cell Subsets.
    Methods Mol Biol. 2020: 143-52.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"