ELISA Synblockis an ELISA buffer designed to avoid false positive results associated with buffers containing animal proteins (e.g. BSA) and reduce non-specific background signals in ELISA assays without the addition of the usuall protein additives.
- Intended Use
- ELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With Tween and synthetic blocking agents, the inert nature of this unique buffer, enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.
Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
N.B. SYNBLOCK is not suitable for use on Immunolon-2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
- Product Form
- Ready to use - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Store at +4oC.
DO NOT FREEZE
- Guaranteed until date of expiry. Please see product label.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ELISA Synblock
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Coat ELISA plate with antibody or antigen as required.
2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.
3. Add 300-400ul of BUF034A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.
4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
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Useful Reagents Available
Application Based External Images
Product Specific References
References for ELISA Synblock
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J Neurochem. 112: 1045-53.
Chambers, M.A. et al. (2009) Performance of TB immunodiagnostic tests in Eurasian badgers (Meles meles) of different ages and the influence of duration of infection on serological sensitivity.
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Thompson, R. et al. (2011) Optimization of the enzyme-linked lectin assay for enhanced glycoprotein and glycoconjugate analysis.
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Abdul, H.M. et al. (2009) Cognitive decline in Alzheimer's disease is associated with selective changes in calcineurin/NFAT signaling.
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Niedowicz DM et al. (2013) Leptin regulates amyloid β production via the γ-secretase complex.
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Martin SB et al. (2014) Synaptophysin and synaptojanin-1 in Down syndrome are differentially affected by Alzheimer's disease.
J Alzheimers Dis. 42 (3): 767-75.
Wilcock, D.M. et al. (2015) Down syndrome individuals with Alzheimer's disease have a distinct neuroinflammatory phenotype compared to sporadic Alzheimer's disease.
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Chinthamani S et al. (2017) Macrophage inducible C-type lectin (Mincle) recognizes glycosylated surface (S)-layer of the periodontal pathogen Tannerella forsythia.
PLoS One. 12 (3): e0173394.
LeVine, H. et al. (2017) Down syndrome: Age-dependence of PiB binding in postmortem frontal cortex across the lifespan
Neurobiology of Aging. Mar 16 [Epub ahead of print]