ELISA General Assay Diluent

ELISA General Assay Diluent

Product Type
Accessory Reagent
Specificity
ELISA General Assay Diluent

Product Code Applications Pack Size List Price Your Price Qty
BUF037A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml
List Price Your Price
BUF037B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 ml
List Price Your Price
BUF037C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1000 ml
List Price Your Price

Intended Use
BUF037A is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum, plasma or cell culture samples. Designed for use in all sandwich ELISA formats, it contains mammalian serum proteins to reduce non-specific interactions between sample proteins and the plate surface.

The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF037A equalizes the matrix, inhibits complement and thrombin activity and contains an antimicrobial agent.
Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.17% Proclin 300
<10% EDTA
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
Proclin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Pack Size: 100 ml
Store at +4°C.

DO NOT FREEZE
Pack Size: 500 ml
Store at +4eg;C.
DO NOT FREEZE.
Pack Size: 1000 ml
Store at +4<°C.
DO NOT FREEZE

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Pipette buffer directly onto the plate, just prior to adding the sample or standard (suggested volume 50 - 100 ul/well).

NB. Since this buffer contains proteins derived from goat, BUF037A is not recommended for ELISA where an anti goat secondary antibody is used.
Pipette buffer directly onto the plate, just prior to adding the sample and standard (suggested volume 50 - 100 ul/well).

NB. Since this buffer contains proteins derived from goat, BUF037B is not recommended for ELISA where an anti goat secondary antibody is used.
Pipette buffer directly onto the plate, just prior to adding the sample and standard (suggested volume 50 - 100 ul/well).
NB. Since this buffer contains proteins derived from goat, BUF037C is not recommended for ELISA where an anti goat secondary antibody is used.

Description Product Code Applications Pack Size List Price Your Price Quantity
ELISA BSA Block BUF032A E 100 ml
List Price Your Price
Description ELISA BSA Block
10x ELISA Wash Buffer BUF031A E 100 ml
List Price Your Price
Description 10x ELISA Wash Buffer
5x ELISA Coating Buffer BUF030A E 100 ml
List Price Your Price
Description 5x ELISA Coating Buffer

BUF037A

155684 157660 158203 158588 159929 161321 163281 164145

BUF037B

170817 170817A

BUF037C

155077 158198 160571 164091

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"