- Intended Use
- BUF037A is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum, plasma or cell culture samples. Designed for use in all sandwich ELISA formats, it contains mammalian serum proteins to reduce non-specific interactions between sample proteins and the plate surface.
The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF037A equalizes the matrix, inhibits complement and thrombin activity and contains an antimicrobial agent.
- Product Form
- Ready to use - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.17% Proclin™ 300
- Store at +4oC.
DO NOT FREEZE
- Guaranteed until date of expiry. Please see product label.
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ELISA General Assay Diluent
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Pipette buffer directly onto the plate, just prior to adding the sample or standard (suggested volume 50 - 100 ul/well).
NB. Since this buffer contains proteins derived from goat, BUF037A is not recommended for ELISA where an anti goat secondary antibody is used.
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Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra