CYTOTRACK™

CytoTrack cell proliferation assay kits are available in four distinct dyes for easy multicolor cell analysis: blue, green, yellow and red. Easily incorporate a cell tracking stain into your multicolor panel.

The proprietary chemistry of CytoTrack dyes enables the resolution of up to ten cell divisions. Each dye is cell permeable and comprises a fluorophore, a fluorescence blocker and a cell-retaining group. Upon entering a live cell, the fluorescence blocker is cleaved by intracellular esterases and the cell-retaining group of the fluorophore reacts with intracellular proteins to create a stable, covalent bond. As the cells divide, the fluorescence intensity is successively halved and each cell divison can be identified.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/500
Flow Cytometry		1/500
Flow Cytometry		1/500
Flow Cytometry		1/500

Instructions For Use

Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 10⁶ cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 10⁶cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.

Instructions For Use

Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 10⁶ cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 10⁶cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.

Instructions For Use

Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 10⁶ cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 10⁶cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.

Instructions For Use

Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 10⁶ cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 10⁶cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.