CYTOTRACK™

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CYTOTRACK™ Blue 403/454 Cell Proliferation Assay Kit

CYTOTRACK™ Green 511/525 Cell Proliferation Assay Kit

CYTOTRACK™ Yellow 542/556 Cell Proliferation Assay Kit

CYTOTRACK™ Red 628/643 Cell Proliferation Assay Kit

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1351202 F 200 Tests
1351203 F 200 Tests
1351204 F 200 Tests
1351205 F 200 Tests
CytoTrack cell proliferation assay kits are available in four distinct dyes for easy multicolor cell analysis: blue, green, yellow and red. Easily incorporate a cell tracking stain into your multicolor panel.

The proprietary chemistry of CytoTrack dyes enables the resolution of up to ten cell divisions. Each dye is cell permeable and comprises a fluorophore, a fluorescence blocker and a cell-retaining group. Upon entering a live cell, the fluorescence blocker is cleaved by intracellular esterases and the cell-retaining group of the fluorophore reacts with intracellular proteins to create a stable, covalent bond. As the cells divide, the fluorescence intensity is successively halved and each cell divison can be identified.

Product Details

Reagents in the Kit
CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl)
Reagents in the Kit
CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl)
Reagents in the Kit
CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl)
Reagents in the Kit
CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl)

Storage Information

Storage
Store at -20oC only

This product is photosensitive and should be protected from light
Storage
Store at -20oC only

This product is photosensitive and should be protected from light
Storage
Store at -20oC only

This product is photosensitive and should be protected from light
Storage
Store at -20oC only

This product is photosensitive and should be protected from light
Shelf Life
Please see label for expiry date
Shelf Life
Please see label for expiry date
Shelf Life
Please see label for expiry date
Shelf Life
Please see label for expiry date

More Information

Regulatory
For research purposes only

Applications of CYTOTRACK™

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/500
Flow Cytometry 1/500
Flow Cytometry 1/500
Flow Cytometry 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.
Instructions For Use
Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.
Instructions For Use
Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.
Instructions For Use
Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.