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CYTOTRACK™ Blue 403/454 Cell Proliferation Assay Kit

Product Type
Accessory Reagent

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CytoTrack cell proliferation assay kits are available in three distinct dyes for easy multicolor cell analysis: blue, green and red. Easily incorporate a cell tracking stain into your multicolor panel.

The proprietary chemistry of CytoTrack dyes enables the resolution of up to ten cell divisions. Each dye is cell permeable and comprises a fluorophore, a fluorescence blocker and a cell-retaining group. Upon entering a live cell, the fluorescence blocker is cleaved by intracellular esterases and the cell-retaining group of the fluorophore reacts with intracellular proteins to create a stable, covalent bond. As the cells divide, the fluorescence intensity is successively halved and each cell divison can be identified.

Reagents In The Kit
CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl)
For research purposes only
Guaranteed until date of expiry. Please see product label.

Store at -20oC only

This product is photosensitive and should be protected from light

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Important: Thaw all components prior to use.

1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

2. Protocol for use in culture medium (for product 1351203) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

3. Incubate at room temperature for 15 mins. Protect from light.

4. Pellet the cells by centrifugation.

5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

6. Resuspend the cell in 500 μl of culture media.

7. Place the cells in the appropriate conditions for cells proliferation.

8. Harvest the cells and stain them for other markers if appropriate.

9. Analyze or sort the cells using a flow cytometer or S3e cell sorter with the appropriate excitation and emission filters.

References for CYTOTRACK™

  1. Perrotta, C. et al. (2018) Nitric Oxide Generated by Tumor-Associated Macrophages Is Responsible for Cancer Resistance to Cisplatin and Correlated With Syntaxin 4 and Acid Sphingomyelinase Inhibition.
    Front Immunol. 9: 1186.
  2. Perrotta, C. et al. (2016) Climacostol reduces tumour progression in a mouse model of melanoma via the p53-dependent intrinsic apoptotic programme.
    Sci Rep. 6: 27281.
  3. Zecchini, S. et al. (2019) Autophagy controls neonatal myogenesis by regulating the GH-IGF1 system through a NFE2L2- and DDIT3-mediated mechanism.
    Autophagy. 15 (1): 58-77.
  4. Saito, Y. et al. (2020) Exercise enhances skeletal muscle regeneration by promoting senescence in fibro-adipogenic progenitors.
    Nat Commun. 11 (1): 889.
  5. Cappello, P. et al. (2016) Anti-α-enolase antibody limits the invasion of myeloid-derived suppressor cells and attenuates their restraining effector T cell response.
    Oncoimmunology. 5 (5): e1112940.
  6. Tario, J.J. et al. (2018) Monitoring Cell Proliferation by Dye Dilution: Considerations for Probe Selection.
    Methods Mol Biol. 1678: 249-99.
  7. Loef, E.J. et al. (2021) Live-Cell Microscopy Reveals That Human T Cells Primarily Respond Chemokinetically Within a CCL19 Gradient That Induces Chemotaxis in Dendritic Cells.
    Front Immunol. 12: 628090.
  8. Wang, X. et al. (2021) Activatable Biomineralized Nanoplatform Remodels the Intracellular Environment of Multidrug-Resistant Tumors for Enhanced Ferroptosis/Apoptosis Therapy.
    Small. : e2102269.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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