CYTOTRACK™


CYTOTRACK™ Blue 403/454 Cell Proliferation Assay Kit
CYTOTRACK™ Green 511/525 Cell Proliferation Assay Kit
CYTOTRACK™ Yellow 542/556 Cell Proliferation Assay Kit
CYTOTRACK™ Red 628/643 Cell Proliferation Assay Kit
- Product Type
- Accessory Reagent
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
1351202 | F | 200 Tests | ||
1351203 | F | 200 Tests | ||
1351204 | F | 200 Tests | ||
1351205 | F | 200 Tests |
The proprietary chemistry of CytoTrack dyes enables the resolution of up to ten cell divisions. Each dye is cell permeable and comprises a fluorophore, a fluorescence blocker and a cell-retaining group. Upon entering a live cell, the fluorescence blocker is cleaved by intracellular esterases and the cell-retaining group of the fluorophore reacts with intracellular proteins to create a stable, covalent bond. As the cells divide, the fluorescence intensity is successively halved and each cell divison can be identified.
Product Details
- Reagents in the Kit
- CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl) - Reagents in the Kit
- CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl) - Reagents in the Kit
- CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl) - Reagents in the Kit
- CytoTrack Dye (4 vials, 50 assays/vial)
DMSO (1 vial, 250 μl)
Storage Information
- Storage
- Store at -20oC only
This product is photosensitive and should be protected from light - Storage
- Store at -20oC only
This product is photosensitive and should be protected from light - Storage
- Store at -20oC only
This product is photosensitive and should be protected from light - Storage
- Store at -20oC only
This product is photosensitive and should be protected from light - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of CYTOTRACK™
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/500 | ||
Flow Cytometry | 1/500 | ||
Flow Cytometry | 1/500 | ||
Flow Cytometry | 1/500 |
- Instructions For Use
- Important: Thaw all components prior to use.
1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.
2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.
Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.
3. Incubate at room temperature for 15 mins. Protect from light.
4. Pellet the cells by centrifugation.
5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.
6. Resuspend the cell in 500 μl of culture media.
7. Place the cells in the appropriate conditions for cells proliferation.
8. Harvest the cells and stain them for other markers if appropriate.
9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters. - Instructions For Use
- Important: Thaw all components prior to use.
1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.
2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.
Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.
3. Incubate at room temperature for 15 mins. Protect from light.
4. Pellet the cells by centrifugation.
5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.
6. Resuspend the cell in 500 μl of culture media.
7. Place the cells in the appropriate conditions for cells proliferation.
8. Harvest the cells and stain them for other markers if appropriate.
9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters. - Instructions For Use
- Important: Thaw all components prior to use.
1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.
2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.
Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.
3. Incubate at room temperature for 15 mins. Protect from light.
4. Pellet the cells by centrifugation.
5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.
6. Resuspend the cell in 500 μl of culture media.
7. Place the cells in the appropriate conditions for cells proliferation.
8. Harvest the cells and stain them for other markers if appropriate.
9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters. - Instructions For Use
- Important: Thaw all components prior to use.
1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.
2.Protocol for use in culture medium (for products 1351203 and 1351204) - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.
Protocol for use with buffer (for products 1351202, 1351203 and 1351205) - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.
3. Incubate at room temperature for 15 mins. Protect from light.
4. Pellet the cells by centrifugation.
5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.
6. Resuspend the cell in 500 μl of culture media.
7. Place the cells in the appropriate conditions for cells proliferation.
8. Harvest the cells and stain them for other markers if appropriate.
9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra