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Brefeldin A Solution

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Figure A. RPE conjugated mouse anti human CD3 (MCA2184PE) and FITC conjugated mouse anti human IFN-gamma on unstimulated human lymphocytes. Figure B. RPE conjugated mouse anti human CD3 (MCA2184PE) and FITC conjugated mouse anti human IFN-gamma on human lymphocytes stimulated with PMA and ionomycin for 5 hours in the presence of brefeldin A (BUF075). Data acquired on the ZE5™ Cell Analyzer.

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Brefeldin A Solution (1000X)

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Accessory Reagent
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Brefeldin A Solution

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Brefeldin A Solution (1000x) is commonly used to enhance intracellular cytokine staining by inhibiting protein transport. This solution causes many of the cytokines to accumulate in the Golgi apparatus and endoplasmic reticulum.

Product Form
1000x concentrate - liquid
Preparation
5 mg/ml solution prior to dilution
Preservative Stabilisers
Dimethyl sulfoxide (DMSO)
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Thaw before use as DMSO freezes at the recommended storage conditions
2. Dilute the Brefeldin A Solution (1000x) to a 1x working concentration using cell culture media
3. Add Brefeldin A Solution (1x) to cell culture for 4-24 hours prior to antibody staining. Do not exceed cell culture time as this may affect cell viability

Note: Aliquot product to avoid repeat freeze-thawing. Time and culture conditions may need to be optimized.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"