Brefeldin A Solution
Brefeldin A Solution (1000x) is commonly used to enhance intracellular cytokine staining by inhibiting protein transport. This solution causes many of the cytokines to accumulate in the Golgi apparatus and endoplasmic reticulum.
Product Details
- Product Form
- 1000x concentrate - liquid
- Preparation
- 5 mg/ml solution prior to dilution
- Preservative Stabilisers
- Dimethyl sulfoxide (DMSO)
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. - Shelf Life
- Please see label for expiry date
More Information
- Regulatory
- For research purposes only
Applications of Brefeldin A Solution
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Thaw before use as DMSO freezes at the recommended storage conditions
2. Dilute the Brefeldin A Solution (1000x) to a 1x working concentration using cell culture media
3. Add Brefeldin A Solution (1x) to cell culture for 4-24 hours prior to antibody staining. Do not exceed cell culture time as this may affect cell viability
Note: Aliquot product to avoid repeat freeze-thawing. Time and culture conditions may need to be optimized.
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