Brefeldin A Solution
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|Brefeldin A Solution (1000x) is commonly used to enhance intracellular cytokine staining by inhibiting protein transport. This solution causes many of the cytokines to accumulate in the Golgi apparatus and endoplasmic reticulum.|
- Product Form
- 1000x concentrate - liquid
- 5 mg/ml solution prior to dilution
- Preservative Stabilisers
- Dimethyl sulfoxide (DMSO)
- For research purposes only
- Guaranteed until date of expiry. Please see product label.
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
- Instructions For Use
- 1. Thaw before use as DMSO freezes at the recommended storage conditions
2. Dilute the Brefeldin A Solution (1000x) to a 1x working concentration using cell culture media
3. Add Brefeldin A Solution (1x) to cell culture for 4-24 hours prior to antibody staining. Do not exceed cell culture time as this may affect cell viability
Note: Aliquot product to avoid repeat freeze-thawing. Time and culture conditions may need to be optimized.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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