Block ACE
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Block Ace is designed as a high-performance blocking reagent for use in immunological assays such as ELISA and Western blotting. It may also be used for dilution of antibodies and for washing procedures in the above assays.
Block Ace has been shown to have superior performance to 1% BSA in blocking in ELISA assays. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves.
For blocking in ELISA applications, we recommend using a 1/4 dilution of the reconstituted solution.
For blocking in Western Blotting applications, we recommend using the reconstituted solution neat.
For washing applications we recommend a 1/10 dilution of the reconstituted solution, and adding Tween 20 to a level of 0.05-0.2% v/v.
For use as a test sample or secondary antibody diluent we recommend a 1/10 dilution of the reconstituted solution.
Block Ace has been shown to have superior performance to 1% BSA in blocking in ELISA assays. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves.
For blocking in ELISA applications, we recommend using a 1/4 dilution of the reconstituted solution.
For blocking in Western Blotting applications, we recommend using the reconstituted solution neat.
For washing applications we recommend a 1/10 dilution of the reconstituted solution, and adding Tween 20 to a level of 0.05-0.2% v/v.
For use as a test sample or secondary antibody diluent we recommend a 1/10 dilution of the reconstituted solution.
Product Details
- Reconstitution
- Reconstitute each 4g sachet in 100ml distilled water.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- Prior to reconstitution, 2 years from date of despatch. After reconstitution, 1 week at 4oC.
More Information
- Regulatory
- For research purposes only
Applications of Block ACE
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/4 | ||
| Western Blotting | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the reagent for use in their own system using appropriate negative/positive controls
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Block ACE
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Faraj SF et al. (2015) Assessment of tumoral PD-L1 expression and intratumoral CD8+ T cells in urothelial carcinoma.
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Park, M.C. et al. (2016) Droplet-based magnetic bead immunoassay using microchannel-connected multiwell plates (μCHAMPs) for the detection of amyloid beta oligomers.
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Kanzaki, H. et al. (2017) Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor.
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Qosa, H. et al. (2015) Extra-virgin olive oil attenuates amyloid-β and tau pathologies in the brains of TgSwDI mice.
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