Block Ace has been shown to have superior performance to 1% BSA in blocking in ELISA assays. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves.
For blocking in ELISA applications, we recommend using a 1/4 dilution of the reconstituted solution.
For blocking in Western Blotting applications, we recommend using the reconstituted solution neat.
For washing applications we recommend a 1/10 dilution of the reconstituted solution, and adding Tween 20 to a level of 0.05-0.2% v/v.
For use as a test sample or secondary antibody diluent we recommend a 1/10 dilution of the reconstituted solution.
- Reconstitute each 4g sachet in 100ml distilled water.
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- For research purposes only
Applications of Block ACE
|Application Name||Verified||Min Dilution||Max Dilution|
Product Specific References
References for Block ACE
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Shinmoto, H. et al. (1988) Production of pentameric hybrid immunoglobulins consisting of IgA and IgM.
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Yamashita, M. et al. (2009) Surgical method to create vocal fold injuries in mice.
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Wilcock, D.M. et al. (2015) Down syndrome individuals with Alzheimer's disease have a distinct neuroinflammatory phenotype compared to sporadic Alzheimer's disease.
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Tarhan, Y.E. et al. (2016) Morphological Changes, Cadherin Switching, and Growth Suppression in Pancreatic Cancer by GALNT6 Knockdown
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Hashitani, H. et al. (2015) Pacemaker role of pericytes in generating synchronized spontaneous Ca2+ transients in the myenteric microvasculature of the guinea-pig gastric antrum.
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Wilcock DM et al. (2015) Down syndrome individuals with Alzheimer's disease have a distinct neuroinflammatory phenotype compared to sporadic Alzheimer's disease.
Neurobiol Aging. 36 (9): 2468-74.
Faraj SF et al. (2015) Assessment of tumoral PD-L1 expression and intratumoral CD8+ T cells in urothelial carcinoma.
Urology. 85 (3): 703.e1-6.
Park, M.C. et al. (2016) Droplet-based magnetic bead immunoassay using microchannel-connected multiwell plates (μCHAMPs) for the detection of amyloid beta oligomers.
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Kanzaki, H. et al. (2017) Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor.
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Qosa, H. et al. (2015) Extra-virgin olive oil attenuates amyloid-β and tau pathologies in the brains of TgSwDI mice.
J Nutr Biochem. 26 (12): 1479-90.