Block Ace has been shown to have superior performance to 1% BSA in blocking in ELISA assays. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves.
For blocking in ELISA applications, we recommend using a 1/4 dilution of the reconstituted solution.
For blocking in Western Blotting applications, we recommend using the reconstituted solution neat.
For washing applications we recommend a 1/10 dilution of the reconstituted solution, and adding Tween 20 to a level of 0.05-0.2% v/v.
For use as a test sample or secondary antibody diluent we recommend a 1/10 dilution of the reconstituted solution.
- Reconstitute each 4g sachet in 100ml distilled water.
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 1 week from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
- For research purposes only
Applications of Block ACE
|Application Name||Verified||Min Dilution||Max Dilution|
Product Specific References
References for Block ACE
Yamashita, M. et al. (2010) Morphological and extracellular matrix changes following vocal fold injury in mice.
Cells Tissues Organs. 192 (4): 262-71.
Head, E. et al. (2010) Amyloid-beta peptide and oligomers in the brain and cerebrospinal fluid of aged canines.
J Alzheimers Dis. 2010;20(2):637-46.
Hara, H. et al. (1990) Enzyme immunoassay for measuring antibodies against skeletal muscle in patients with myasthenia gravis.
Clin Chem. 36 (11): 1967-9.
Shinmoto, H. et al. (1988) Production of pentameric hybrid immunoglobulins consisting of IgA and IgM.
Agric. Biol. Chem. 52:2653-2654.
Ahmed, R.R. et al. (2010) BACE1 and BACE2 enzymatic activities in Alzheimer's disease.
J Neurochem. 112: 1045-53.
Matsui, T.S. et al. (2011) Non-muscle myosin II induces disassembly of actin stress fibres independently of myosin light chain dephosphorylation.
Interface Focus. 1 (5): 754-66.
Zhang, B. et al. (2012) The microtubule-stabilizing agent, epothilone D, reduces axonal dysfunction, neurotoxicity, cognitive deficits, and Alzheimer-like pathology in an interventional study with aged tau transgenic mice.
J Neurosci. 32 (11): 3601-11.
Fitz, N.F. et al. (2012) Abca1 Deficiency Affects Alzheimer's Disease-Like Phenotype in Human ApoE4 But Not in ApoE3-Targeted Replacement Mice.
J Neurosci. 32: 13125-36.
Habara, P. et al. (2012) Novel flow cytometric method for the detection of podocalyxin-positive elements in urine of patients with glomerulonephritides - first promising results.
Folia Biol (Praha). 58: 57-63.
Yamashita, M. et al. (2009) Surgical method to create vocal fold injuries in mice.
Ann Otol Rhinol Laryngol. 118: 131-8.
Kim, J.A. et al. (2015) Magnetic bead droplet immunoassay of oligomer amyloid β for the diagnosis of Alzheimer's disease using micro-pillars to enhance the stability of the oil-water interface.
Biosens Bioelectron. 67: 724-32.
Wilcock, D.M. et al. (2015) Down syndrome individuals with Alzheimer's disease have a distinct neuroinflammatory phenotype compared to sporadic Alzheimer's disease.
Neurobiol Aging. 36 (9): 2468-74.
Tarhan, Y.E. et al. (2016) Morphological Changes, Cadherin Switching, and Growth Suppression in Pancreatic Cancer by GALNT6 Knockdown
Neoplasia 18: 265-72.
Hashitani, H. et al. (2015) Pacemaker role of pericytes in generating synchronized spontaneous Ca2+ transients in the myenteric microvasculature of the guinea-pig gastric antrum.
Cell Calcium. 58 (5): 442-56.
Wilcock DM et al. (2015) Down syndrome individuals with Alzheimer's disease have a distinct neuroinflammatory phenotype compared to sporadic Alzheimer's disease.
Neurobiol Aging. 36 (9): 2468-74.
Faraj SF et al. (2015) Assessment of tumoral PD-L1 expression and intratumoral CD8+ T cells in urothelial carcinoma.
Urology. 85 (3): 703.e1-6.
Park, M.C. et al. (2016) Droplet-based magnetic bead immunoassay using microchannel-connected multiwell plates (μCHAMPs) for the detection of amyloid beta oligomers.
Lab Chip. 16 (12): 2245-53.
Kanzaki, H. et al. (2017) Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor.
Biochim Biophys Acta. 1862 (5): 452-462.
Qosa, H. et al. (2015) Extra-virgin olive oil attenuates amyloid-β and tau pathologies in the brains of TgSwDI mice.
J Nutr Biochem. 26 (12): 1479-90.
Gibbons, G.S. et al. (2020) Conformation-selective tau monoclonal antibodies inhibit tau pathology in primary neurons and a mouse model of Alzheimer's disease.
Mol Neurodegener. 15 (1): 64.
Ling, C. et al. (2019) Differentiated fibrocytes assume a functional mesenchymal phenotype with regenerative potential.
Sci Adv. 5 (5): eaav7384.
Marotta, N.P. et al. (2021) Alpha-synuclein from patient Lewy bodies exhibits distinct pathological activity that can be propagated in vitro.
Acta Neuropathol Commun. 9 (1): 188.
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