Aqueous Mounting Medium
Immunohistochemical staining of formalin fixed, paraffin embedded murine colonic tissue sections for zonulin-1 and occludin using Permanent aqueous mounting medium (BUF058) as a mountant.
Image caption:
Bl 7952 induces upregulation of zonulin-1 and occludin in colon. Mice were treated with Bl 7952 (n=10), with Bl 372 (n=8) or with PBS (n=10) on ten consecutive days or were left untreated (Naïve; n = 5). Colonic inflammation was induced by addition of 2.5% (w/v) DSS in the drinking water for 7 days. (A) Immunohistochemical detection of occludin and zonulin-1 proteins on representative paraffin-embedded sections of colon. (B) Representative western blotting of occludin and zonulin-1 proteins in the colonic mucosa. Expression of β-actin was used as internal control. (C) Evaluation of intestinal permeability by FITC-dextran. Serum levels of 4.0-kDa FITC-dextran were measured in naïve controls (n=5), PBS/DSS-treated (n=5) and Bl 7952/DSS-treated mice (n=5) 5 hours after its intragastric administration. Data are express as mean ± SEM. Unpaired Student's t-test was used for comparison of Bl 7952/DSS group vs. control PBS/DSS group (**p < 0.01).
From: Srutkova D, Schwarzer M, Hudcovic T, Zakostelska Z, Drab V, Spanova A, et al. (2015) Bifidobacterium longum CCM 7952 Promotes Epithelial Barrier Function and Prevents Acute DSS-Induced Colitis in Strictly Strain-Specific Manner.
PLoS ONE 10(7): e0134050.
Immunohistochemical staining of formalin fixed, paraffin embedded human liver tissue sections for microsomal triglyceride transfer protein using Permanent aqueous mounting medium (BUF058) as a mountant.
Image caption:
MTP associates with lipid droplets in “fatty” human liver. Sections of control and “fatty” human liver were stained with antibody to MTP and DAB-conjugated secondary antibody. Immunoreactive product was found throughout hepatocytes as previously reported for mouse liver [21], and it was especially prominent on lipid droplets (arrows).
From: Love JD, Suzuki T, Robinson DB, Harris CM, Johnson JE, Mohler PJ, et al. (2015) Microsomal Triglyceride Transfer Protein (MTP) Associates with Cytosolic Lipid Droplets in 3T3-L1 Adipocytes.
PLoS ONE 10(8): e0135598.
Permanent Aqueous Mounting Medium used for mounting of Shistosoma japonicum worm sections for fluorescence microscopy.
Image caption:
Detection of mouse IgG binding on the surface of murine adult S.japonicum.
(a) Detection of mouse IgG on the surface of whole adult worms using laser confocal microscopy. F-actin was labeled with Alexa Fluor 488 phalloidin (green) and mouse IgG was detected with Alexa Fluor 555 donkey anti-mouse IgG (red) on the surface of both male (right) and female (left) adult worms. (b) Detection of mouse IgG (in red) on the surface of frozen sections of schistosome using fluorescence microscopy. (c) Detection of mouse IgG (in red) on the surface of a frozen section of adult blood flukes using laser confocal microscopy. DIC: differential interference contrast.
From: Wu C, Hou N, Piao X, Liu S, Cai P, Xiao Y, Chen Q. Non-immune immunoglobulins shield Schistosoma japonicum from host immunorecognition.
Sci Rep. 2015 Aug24;5:13434.
Permanent aqueous mounting medium is a unique aqueous-based mounting medium designed for the permanent preservation of tissue sections and cell smears which have been immunohistochemically stained with chromogens which cannot be dehydrated with organic solvents.
This mounting media is suitable for use with peroxidase and alkaline phosphatase chromogens, Fast red, BCIP/NBT, BCIP/INT, Aminoethylycarbazole (AEC) and DAB/DAB with cobalt and nickel chromogens, and is also compatible Nuclear fast red (NFR) counterstains.
Permanent aqueous mounting medium is not suitable for use with Haematoxylin and Eosin (H and E), Methylene blue or Methyl green counterstains. Use is also not advised on frozen tissue sections with a high lipid content such as brain, or tissue sections undergoing necrosis.
This mounting media is suitable for use with peroxidase and alkaline phosphatase chromogens, Fast red, BCIP/NBT, BCIP/INT, Aminoethylycarbazole (AEC) and DAB/DAB with cobalt and nickel chromogens, and is also compatible Nuclear fast red (NFR) counterstains.
Permanent aqueous mounting medium is not suitable for use with Haematoxylin and Eosin (H and E), Methylene blue or Methyl green counterstains. Use is also not advised on frozen tissue sections with a high lipid content such as brain, or tissue sections undergoing necrosis.
Product Details
- Preservative Stabilisers
- Pack Size: 30 ml0.09% Sodium Azide (NaN3)Pack Size: 100 ml, 250 ml0.09% Sodium Azide (NaN3)
Storage Information
- Storage
- Store at room temperature (+25oC). For long term storage recommend 2-8oC. DO NOT FREEZE.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of Aqueous Mounting Medium
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Ensure product is at room temperature before use.
Do not use non-polar solvent on samples prior to applying this medium.
1. Rinse slide for mounting with distilled or deionized water, touch-dry slide edges on paper towel and place slide on flat surface.
2. Invert vial and open dropper to remove air bubbles.
3. Apply 3-4 drops of mounting medium directly onto specimen and tilt slide back and forth to distribute evenly. A plastic pipette tip may also be used for spreading, taking care not to damage specimen. Remove excess medium by touching slide edges on paper towel.
4. Leave slide to stand for 1-2 hours at room temperature (25oC). Alternatively slide can be heated for 40-60 minutes at 37-40oC.
Mounted slide can now be visualized under a microscope:
1. For oil immersion lens, the oil can be dropped directly onto the mounted specimen, once the mounting medium has dried. A coverslip is not required. The oil can be later removed by wiping with Kim wipes.
OR
2. For oil immersion lens, apply 1-2 drops of preferred organic mounting medium on top of dried mounted specimen and cover with coverslip.
To remove coverslip or mounting medium, soak the slide for 5-10 minutes in warm water (37oC) until medium is dissolved. Give slide final rinse in warm water. Remount if required.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
References for Aqueous Mounting Medium
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Wheeler, J.L. et al. (2013) Novel cellular targets of AhR underlie alterations in neutrophilic inflammation and inducible nitric oxide synthase expression during influenza virus infection.
J Immunol. 190 (2): 659-68. -
Mbita, Z. et al. (2015) The association of RBBP6 variant 3 expressions with apoptosis in human immunodeficiency virus-associated nephropathy (HIVAN).
Exp Mol Pathol. 99 (1): 74-80. -
Brunnström, Å et al. (2015) On the biosynthesis of 15-HETE and eoxin C4 by human airway epithelial cells.
Prostaglandins Other Lipid Mediat. 121 (Pt A): 83-90. -
Srutkova, D. et al. (2015) Bifidobacterium longum CCM 7952 Promotes Epithelial Barrier Function and Prevents Acute DSS-Induced Colitis in Strictly Strain-Specific Manner.
PLoS One. 10 (7): e0134050. -
Love, J.D. et al. (2015) Microsomal Triglyceride Transfer Protein (MTP) Associates with Cytosolic Lipid Droplets in 3T3-L1 Adipocytes.
PLoS One. 10 (8): e0135598. -
Wu C et al. (2015) Non-immune immunoglobulins shield Schistosoma japonicum from host immunorecognition.
Sci Rep. 5: 13434. -
Dlamini, Z. et al. (2019) RBBP6 Is Abundantly Expressed in Human Cervical Carcinoma and May Be Implicated in Its Malignant Progression.
Biomark Cancer. 11: 1179299X19829149.
