Instructions For UseCaution:
Wear gloves, safety glasses/face protection, and protective clothing to avoid direct contact of ReadiDrop solutions with skin and eyes.
1. Resuspend 1 x 106
cells in 500 μl flow cytometry buffer (1x phosphate buffered saline with 3% BSA). The cell concentration is now 2 x 106
2. Add one to two drop of ReadiDrop 7-AAD to the cell suspension.
3. Vortex briefly.
4. Incubate for 1-10 min at room temperature. Note:
Adding one drop to a 500 µl cell suspension produces a final dye concentration of 1 μg/ml. This is typically sufficient to stain 1 x 106
cells. If one drop is not sufficient for your cell type, adding a second drop may improve staining efficacy. When working with a larger volume of cells, scale ReadiDrop solution amount accordingly.
5. Analyze cells by flow cytometry.
- Detect 7-AAD signal in FL3 on Bio-Rad's S3e Cell Sorter. Otherwise, select the most appropriate FL channel on your particular instrument.