DC057 is suitable for use as a negative control for the measurement of non-specific binding of rat monoclonals of isotype IgG2a and IgG1 to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.
Suitable for use as negative control for human, pig, dog and mouse.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitute with 0.5 ml distilled water
Bovine Serum Albumin
Approx. Protein Concentrations
FITC reagent: IgG2a (RAT) RPE reagent: IgG1 (RAT)
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
12 months from date of reconstitution
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul to label 106 cells or 100ul whole blood
Histology Positive Control Tissue
Instructions For Use
Additional Rat IgG2a/IgG1 Negative Control Antibody Formats