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Phosphorylase B

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  • Native Rabbit Phosphorylase B
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7370-9902Edatasheet pdfdatasheet pdf25 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Native Rabbit Phosphorylase B is a member of the protein kinase superfamily. Phosphorylases catalyse the breakdown of glycogen in liver and muscle. The phosphorylase of skeletal muscle occurs in two forms, the active form (phosphorylase-a) and a much less active form (phosphorylase b).
      Phosphorylase b has a molecular weight of approximately 185kDa.
    • Intended Use
    • Target Species
    • Product Form
      Purified enzyme from rabbit skeletal muscle - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present
    • Activity
      55-60 U/mg protein

      One unit will liberate one µM of glucose-1-phosphate from glycogen and orthophosphate per minute at 37ºC and pH 6.8.
    • Purity
      Protein: 95%
    • Approx. Protein Concentrations
      5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Phosphorylase B Formats

    Formats Applications Sizes available
    Phosphorylase B : Purified E 25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Sheep anti Rabbit Phosphorylase B7370-98080.5 mlE, WB

          Recommended Positive Controls

            Histology Controls

              Further Reading

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