Quantum™ FITC-5 MESF

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  • Quantum™ FITC-5 MESF
  • Quantum™ FITC-5 MESF
  • Quantum™ FITC-5 MESF
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    FCSC555BFdatasheet pdfdatasheet pdf100 Tests
    FCSC555B
    FCSC555AFdatasheet pdfdatasheet pdf20 Tests
    FCSC555A
    FCSC555CFdatasheet pdfdatasheet pdf280 Tests
    FCSC555C
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • FCSC555A is comprised of five calibrated fluorescent populations, with different levels of FITC fluorescence intensity, and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9μm). The excitation and emission spectra of the microspheres match those of cell samples labeled with FITC.
    • Intended Use
      Pack Size: 100 TestsFCSC555B is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 280 TestsFCSC555C is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 20 TestsFCSC555A is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit has been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 100 TestsFCSC555B is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 280 TestsFCSC555C is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 20 TestsFCSC555A is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit has been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 100 TestsFCSC555B is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 280 TestsFCSC555C is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
      Pack Size: 20 TestsFCSC555A is used in the quantitation of FITC fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

      This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the populations in the kit has been calibrated against solutions of laser grade fluorescent dye in units of MESF FITC per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
      1) quantitation of fluorescence intensity of samples in terms of MESF;
      2) determination of instrument fluorescence detection threshold;
      3) determination of instrument linearity;
      4) data comparison over time and between multiple instruments.
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.05% Sodium Azide (NaN3)
      0.01% Tween 80 (unlabeled only)
      0.05% Sodium Azide (NaN3)
      0.01% Tween 80 (unlabeled only)
      0.05% Sodium Azide (NaN3)
      0.01% Tween 80 (unlabeled only)
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Pack Size: 100 Tests1 x 5ml bottle of unlabeled microbeads
      5 x 5ml bottles of labeled microbeads
      Pack Size: 280 Tests2 x 7ml bottles of unlabeled microbeads
      10 x 7ml bottles of labeled microbeads
      Pack Size: 20 Tests1 x 1ml bottle of unlabeled microbeads
      5 x 1ml bottles of labeled microbeads
      Pack Size: 100 Tests1 x 5ml bottle of unlabeled microbeads
      5 x 5ml bottles of labeled microbeads
      Pack Size: 280 Tests2 x 7ml bottles of unlabeled microbeads
      10 x 7ml bottles of labeled microbeads
      Pack Size: 20 Tests1 x 1ml bottle of unlabeled microbeads
      5 x 1ml bottles of labeled microbeads
      Pack Size: 100 Tests1 x 5ml bottle of unlabeled microbeads
      5 x 5ml bottles of labeled microbeads
      Pack Size: 280 Tests2 x 7ml bottles of unlabeled microbeads
      10 x 7ml bottles of labeled microbeads
      Pack Size: 20 Tests1 x 1ml bottle of unlabeled microbeads
      5 x 1ml bottles of labeled microbeads
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
    • Shelf Life
      See packaging for expiry date
      See packaging for expiry date
      See packaging for expiry date
    • Acknowledgements
      Quantum™ is a trademark of Bangs Laboratories, INC.
      Quantum™ is a trademark of Bangs Laboratories, INC.
      Quantum™ is a trademark of Bangs Laboratories, INC.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC555C.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC555B.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC555A.pdf

    Additional Quantum™ FITC-5 MESF Formats

    Formats Applications Sizes available
    Quantum™ FITC-5 MESF : Flow Cytometry Calibration Reagent F 20 Tests | 100 Tests | 280 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Quantum™ Simply Cellular® Mouse IgGFCSC815C280 TestsF
          FCSC815C
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Quantum™ Simply Cellular® Mouse IgGFCSC815B100 TestsF
          FCSC815B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Quantum™ Simply Cellular® Mouse IgGFCSC815A20 TestsF
          FCSC815A

          Recommended Positive Controls

            Histology Controls

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