Yellow Fever Virus

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Recombinant Yellow Fever Virus NS1 Protein

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PIP052A E 100 µg
Recombinant yellow fever virus NS1 protein is produced as a hexamer in the human 293 cell line. This product has been purified and retains its native folding state and post-translational modifications providing optimal antigenicity. Research indicates that the hexamer is the biologically active form of the NS1 antigen and therefore involved in the pathogenesis of yellow fever virus (YFV).

Yellow fever virus belongs to the Flaviviridae family and like closely related viruses, such as West Nile virus, is an abovirus utilizing various mosquito species as vectors. YFV appears to be restricted naturally to human and other primates but can be experimentally induced in other mammalian species. In recent years an increasing number cases of YFV infection have been reported.

Originating in Africa, YFV has been introduced to tropical regions of South America and periodic outbreaks have historically occurred in both Europe and North America. According to the World Health Organization (WHO), it has been estimated that approximately 200,000 people contract YFV annually with around 30,000 cases resulting in fatality, the majority of these on the African continent.

This product may be used in research to investigate vaccine development (Bonaldo, M.C. et al. 2014) or in research into the development of assays to detect YFV infection (Ding, X.X,et al. 2014).

Product Details

Target Species
Viral
Product Form
Purified recombinant protein - liquid
Preparation
Recombinant yellow fever virus NS1 protein, sequence strain 17D, expressed in 293 human cells
Buffer Solution
Dulbecco's phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Approx. Protein Concentrations
Approximate protein concentration 0.48 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Yellow Fever Virus

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Ding, X.X. et al. (2014) Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1.
    PLoS One 9(10): e108623.
  2. Bonaldo, M.C. et al. (2014) The yellow fever 17D virus as a platform for new live attenuated vaccines.
    Hum Vaccin Immunother. 10(5): 1256-65.