TNF alpha is produced by activated macrophages, monocytes, endothelial cells, fibroblast and both T and B lymphocytes. Production of ovine TNF alpha can be induced by a variety of factors, dependant upon cell type, and includes bacterial toxins, IL-1, PDGF, IFN beta, NGF, oncostatin M and viral infections. TNF alpha functions in the regulation of immune cells, is able to induce fever, apoptotic cell death, sepsis, cacheria, inflammation and to inhibit tumorigenesis and viral replication.
- Target Species
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 17.2 kDa
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- For research purposes only
Applications of TNF Alpha
|Application Name||Verified||Min Dilution||Max Dilution|