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Pyruvate Kinase

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Native Rabbit Pyruvate Kinase

Product Type
Purified Protein
Specificity
Pyruvate Kinase

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7895-0104
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Target Species
Rabbit
Product Form
Semi purified enzyme from rabbit muscle - lyophilized
Reconstitution
Reconstitute with sterile distilled water. For long term storage, addition of a preservative is recommended.
Preservative Stabilisers
None present
Activity
375 U/mg solid
500 U/mg protein

1 unit liberates 1 micromole pyruvate per minute from PEP in the presence of ADP at 30oC and pH7.6.
Approx. Protein Concentrations
75%
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

UniProt
P11974
Entrez Gene
LOC100008676
GO Terms
GO:0000287 magnesium ion binding
GO:0004743 pyruvate kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006096 glycolysis
GO:0030955 potassium ion binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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