Pyruvate Kinase

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Native Rabbit Pyruvate Kinase

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7895-0104 E 50 kU

Product Details

Target Species
Rabbit
Product Form
Semi purified enzyme from rabbit muscle - lyophilised
Reconstitution
Reconstitute with sterile distilled water. For long term storage, addition of a preservative is recommended.
Preservative Stabilisers
None present
Activity
375 U/mg solid
500 U/mg protein

1 unit liberates 1 micromole pyruvate per minute from PEP in the presence of ADP at 30oC and pH7.6.
Approx. Protein Concentrations
75%

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11974 Related reagents
Entrez Gene
LOC100008676 Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0004743 pyruvate kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006096 glycolysis
GO:0030955 potassium ion binding
Regulatory
For research purposes only

Applications of Pyruvate Kinase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.