TNF Alpha
Recombinant Pig TNF Alpha
- Product Type
- Recombinant Protein
- Specificity
- TNF Alpha
Antibody Quality and Performance Guarantee
| Recombinant Pig tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), corresponds to aa 77-232 of native pig TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified. Pig TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the porcine inflammatory response. This product may be used as a standard in immunoassays. |
- Target Species
- Pig
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 16.9 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for TNF Alpha
-
Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
Res Vet Sci. 131: 266-300.
- Synonyms
- TNFSF2
- UniProt
- P23563
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
PPP029
149198
153017
161241
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with TNF ALPHA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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