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Thyroglobulin

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Native Human Thyroglobulin gallery image 1
Sandwich ELISA analysis of human thyroglobulin using Mouse anti Human thyroglobulin , clone 5F9 (8900-0700) as a capture reagent and biotinylated Mouse anti Human thyroglobulin, clone 5E6 (8900-0730) as a detection reagent with purified human thyroglobulin as antigen (PHP236) for production of a standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples (green and blue) and plasma samples (orange and red) are shown

Native Human Thyroglobulin

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP236 E 1 mg

Product Details

Target Species
Human
Product Form
Purified protein from human thyroid glands - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Purity
SDS PAGE: >98%, ultra-pure, IgG low
Approx. Protein Concentrations
1.0 mg/ml
Molecular Weight
660 kDa, confirmed by chromatography and electrophoresis. Under certain conditions the subunit molecular weight may be observed (330 kDa).

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
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TG Related reagents
Regulatory
For research purposes only

Applications of Thyroglobulin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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