TGF Beta 1

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Recombinant Human TGF Beta 1

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP143B FN 10 µg
Transforming growth factor beta-1 (TGF beta 1) is a homodimeric protein that regulates the proliferation and differentiation of normal and transformed cells. TGF beta 1 is the most abundant isoform which is secreted by most cell types.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 100ul distilled water. Further dilutions should be made in buffer containing a carrier protein such as 0.1% BSA.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX.
Preparation
Recombinant protein prepared from CHO cells.
Preservative Stabilisers
None present
Activity
The ED50, determined by the ability of TGF beta 1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤0.05 ng/ml, corresponding to a specific activity of ≥2 x 107 units/mg
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
25.0 kDa (2x identical 112 amino acid polypeptide chains)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P01137 Related reagents
Entrez Gene
TGFB1 Related reagents
GO Terms
GO:0016049 cell growth
GO:0001933 negative regulation of protein phosphorylation
GO:0002062 chondrocyte differentiation
GO:0002244 hemopoietic progenitor cell differentiation
GO:0002248 connective tissue replacement involved in inflammatory response wound healing
GO:0002576 platelet degranulation
GO:0005114 type II transforming growth factor beta receptor binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005796 Golgi lumen
GO:0006611 protein export from nucleus
GO:0006754 ATP biosynthetic process
GO:0006917 induction of apoptosis
GO:0007050 cell cycle arrest
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007183 SMAD protein complex assembly
GO:0007184 SMAD protein import into nucleus
GO:0007435 salivary gland morphogenesis
GO:0048535 lymph node development
GO:0008083 growth factor activity
GO:0008156 negative regulation of DNA replication
GO:0010718 positive regulation of epithelial to mesenchymal transition
GO:0010742 macrophage derived foam cell differentiation
GO:0010763 positive regulation of fibroblast migration
GO:0010800 positive regulation of peptidyl-threonine phosphorylation
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0016202 regulation of striated muscle tissue development
GO:0016563 transcription activator activity
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0019049 evasion of host defenses by virus
GO:0019058 viral infectious cycle
GO:0022408 negative regulation of cell-cell adhesion
GO:0030168 platelet activation
GO:0030308 negative regulation of cell growth
GO:0030501 positive regulation of bone mineralization
GO:0031093 platelet alpha granule lumen
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0032355 response to estradiol stimulus
GO:0032570 response to progesterone stimulus
GO:0032740 positive regulation of interleukin-17 production
GO:0032801 receptor catabolic process
GO:0032967 positive regulation of collagen biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0035307 positive regulation of protein dephosphorylation
GO:0043406 positive regulation of MAP kinase activity
GO:0043536 positive regulation of blood vessel endothelial cell migration
GO:0043537 negative regulation of blood vessel endothelial cell migration
GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity
GO:0043932 ossification involved in bone remodeling
GO:0045216 cell-cell junction organization
GO:0045599 negative regulation of fat cell differentiation
GO:0045930 negative regulation of mitotic cell cycle
GO:0046732 active induction of host immune response by virus
GO:0048298 positive regulation of isotype switching to IgA isotypes
GO:0050680 negative regulation of epithelial cell proliferation
GO:0050714 positive regulation of protein secretion
GO:0050921 positive regulation of chemotaxis
GO:0051781 positive regulation of cell division
GO:0051897 positive regulation of protein kinase B signaling cascade
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060391 positive regulation of SMAD protein import into nucleus
GO:0070723 response to cholesterol
Regulatory
For research purposes only

Applications of TGF Beta 1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for TGF Beta 1

  1. Brittain, J. et al. (2001) Activation of sickle red blood cell adhesion via integrin associated protein/CD47 - induced signal transduction.
    J. Clin. Invest. 107: 1555 - 1562.
  2. Chaudhary, N.I. et al. (2007) Inhibition of PDGF, VEGF and FGF signalling attenuates fibrosis.
    Eur Respir J. 29: 976-85.
  3. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  4. Sándor K et al. (2016) Transcriptional control of transglutaminase 2 expression in mouse apoptotic thymocytes.
    Biochim Biophys Acta. Jun 1. pii: S1874-9399(16)30121-3. [Epub ahead of print]