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TGF Alpha

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Recombinant Human TGF Alpha

Product Type
Recombinant Protein
Specificity
TGF Alpha

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PHP037
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Recombinant human TGFα is a 5.5kDa peptide corresponding to full-length mature human transforming growth factor alpha. TGFα is an EGF-related growth factor that stimulates the proliferation of epidermal and epithelial cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation
Recombinant protein expressed in E. coli.
Source
E.coli
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
>5 x 106 Units/mg
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Protein Molecular Weight
5.5kD (50 Amino Acid Sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.5 25ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human TGFα may be used as a standard for ELISA applications with purifed human TGFα antibody (AHP284G).
Western Blotting
Recombinant human TGFα may be used as the positive control for Western Blotting applications with purified human TGFα antibody (AHP284G).

Synonyms
Transforming Growth Factor Alpha
UniProt
P01135
Entrez Gene
TGFA
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0016021 integral to membrane
GO:0004708 MAP kinase kinase activity
GO:0004871 signal transducer activity
GO:0005154 epidermal growth factor receptor binding
GO:0045741 positive regulation of epidermal growth factor receptor activity
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0045840 positive regulation of mitosis
GO:0050679 positive regulation of epithelial cell proliferation
GO:0051781 positive regulation of cell division

PHP037

166427

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