SFRP1

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Recombinant Human SFRP1

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP253 FN 20 µg
Recombinant Human SFRP1 antigen produced in Hela cells corresponds to human SFRP1 (Secreted Frizzled-Related Protein-1), a widely expressed multi-functional secreted protein, and member of the sFRP family. SFRP1acts as a modulator of Wnt signalling, playing an important role in embryonic development as well as proliferation, differentiation, and apoptosis in adult tissues. SFRP1 acts as a promoter of Wnt-induced effects at lower concentrations, and as a negative regulator at high concentrations.

SFRP1 has been implicated as a tumour suppressor protein in several human malignancies, including colorectal, breast, bladder, and ovarian cancer.

Recombinant Human SFRP1 is a 283 amino acid residue glycosylated protein containing a cystene-rich Frizzled homologous domain.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant SFRP1 expressed in HeLa cells
Preservative Stabilisers
None present
Activity
Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells, with an expected ED50 of 0.3–0.5 μg/ml.
Purity
>97% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Molecular Weight
283 amino acid residue glycosylated protein containing a cystene-rich Frizzled homologous domain.
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
Q8N474 Related reagents
Entrez Gene
SFRP1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004197 cysteine-type endopeptidase activity
GO:0001649 osteoblast differentiation
GO:0001654 eye development
GO:0001657 ureteric bud development
GO:0001843 neural tube closure
GO:0001944 vasculature development
GO:0002244 hemopoietic progenitor cell differentiation
GO:0005829 cytosol
GO:0042813 Wnt receptor activity
GO:0005109 frizzled binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0007420 brain development
GO:0008144 drug binding
GO:0008201 heparin binding
GO:0061133 endopeptidase activator activity
GO:0008584 male gonad development
GO:0008585 female gonad development
GO:0042802 identical protein binding
GO:0009267 cellular response to starvation
GO:0042493 response to drug
GO:0009950 dorsal/ventral axis specification
GO:0009986 cell surface
GO:0010551 regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010564 regulation of cell cycle process
GO:0010719 negative regulation of epithelial to mesenchymal transition
GO:0014034 neural crest cell fate commitment
GO:0014070 response to organic cyclic compound
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0017147 Wnt-protein binding
GO:0022601 menstrual cycle phase
GO:0030165 PDZ domain binding
GO:0030279 negative regulation of ossification
GO:0030307 positive regulation of cell growth
GO:0030308 negative regulation of cell growth
GO:0030336 negative regulation of cell migration
GO:0030514 negative regulation of BMP signaling pathway
GO:0032582 negative regulation of gene-specific transcription
GO:0032855 positive regulation of Rac GTPase activity
GO:0033689 negative regulation of osteoblast proliferation
GO:0035019 somatic stem cell maintenance
GO:0043065 positive regulation of apoptosis
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0043508 negative regulation of JUN kinase activity
GO:0044344 cellular response to fibroblast growth factor stimulus
GO:0045578 negative regulation of B cell differentiation
GO:0045600 positive regulation of fat cell differentiation
GO:0045668 negative regulation of osteoblast differentiation
GO:0045671 negative regulation of osteoclast differentiation
GO:0045765 regulation of angiogenesis
GO:0045880 positive regulation of smoothened signaling pathway
GO:0046676 negative regulation of insulin secretion
GO:0046851 negative regulation of bone remodeling
GO:0048147 negative regulation of fibroblast proliferation
GO:0048546 digestive tract morphogenesis
GO:0050679 positive regulation of epithelial cell proliferation
GO:0050680 negative regulation of epithelial cell proliferation
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0051496 positive regulation of stress fiber assembly
GO:0051894 positive regulation of focal adhesion assembly
GO:0060070 canonical Wnt receptor signaling pathway
GO:0060071 Wnt receptor signaling pathway, planar cell polarity pathway
GO:0060218 hemopoietic stem cell differentiation
GO:0060346 bone trabecula formation
GO:0060527 prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
GO:0060687 regulation of branching involved in prostate gland morphogenesis
GO:0060766 negative regulation of androgen receptor signaling pathway
GO:0071305 cellular response to vitamin D
GO:0071347 cellular response to interleukin-1
GO:0071356 cellular response to tumor necrosis factor
GO:0071380 cellular response to prostaglandin E stimulus
GO:0071392 cellular response to estradiol stimulus
GO:0071456 cellular response to hypoxia
GO:0071504 cellular response to heparin
GO:0071560 cellular response to transforming growth factor beta stimulus
GO:0071773 cellular response to BMP stimulus
GO:0090246 convergent extension involved in somitogenesis
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
GO:2000052 positive regulation of non-canonical Wnt receptor signaling pathway
GO:2000054 negative regulation of Wnt receptor signaling pathway involved in dorsal/ventral axis specification
GO:2000080 negative regulation of canonical Wnt receptor signaling pathway involved in controlling type B pancreatic cell proliferation
GO:2000117 negative regulation of cysteine-type endopeptidase activity
Regulatory
For research purposes only

Applications of SFRP1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.