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Serum Albumin

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Sandwich ELISA analysis of albumin binding using Mouse anti Human albumin (0300-0080) as a capture reagent and biotinylated Sheep anti Human albumin (AHP1790) as a detection reagent with human albumin purified from urine (PHP149) as antigen. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma sample is displayed at 30000 (Green) and 60000 (Red) fold dilutions.

Purified Human Albumin

Product Type
Purified Protein
Specificity
Serum Albumin

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Product Code Applications Pack Size List Price Your Price Qty
PHP149
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SDS Safety Datasheet SDS
E IY 1 mg loader
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Purified human albumin is a ~66 kDa protein that binds and transports steroids, fatty acids, metal ions and thyroid hormones. Albumin accounts for approximately 50% of blood serum protein.

Target Species
Human
Product Form
Purified human serum albumin - lyophilized
Reconstitution
Reconstitute with phosphate buffer pH>7.0 containing 0.15M NaCl
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preparation
Purified albumin from human urine
Preservative Stabilisers
None present
Purity
>99% by SDS PAGE
Protein Molecular Weight
66 kDa
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoassay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Serum Albumin

  1. Nicholson, J.P. et al. (2000) The role of albumin in critical illness.
    Br J Anaesth. 85 (4): 599-610.

Synonyms
HSA
UniProt
P02768
Entrez Gene
ALB

PHP149

150564 151951 158848 159086 161142 171123

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