RANTES

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Recombinant Human RANTES

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP062 E FN WB 20 µg
RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, is a 68 amino acid CC- chemokine which acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant RANTES expressed in E. coli.
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution
Protein Molecular Weight
7.8kD (68 Amino acid sequence)
Endotoxin Level
<0.1 ng/mg

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution

More Information

UniProt
P13501 Related reagents
Entrez Gene
CCL5 Related reagents
GO Terms
GO:0009615 response to virus
GO:0007155 cell adhesion
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006874 cellular calcium ion homeostasis
GO:0006887 exocytosis
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0006979 response to oxidative stress
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0042056 chemoattractant activity
GO:0045071 negative regulation of viral genome replication
Regulatory
For research purposes only

Applications of RANTES

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2ng/well 0.4ng/well
Functional Assays 10ng/ml
Western Blotting 1.5ng/lane 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate positive/negative controls.
ELISA
PHP062 may be used as an ELISA standard for both AHP340G and AHP340B.
Western Blotting
PHP062 may be used as a positive control in western blotting with either AHP340G or AHP340B.