PDGF

100% Secure

Recombinant Human PDGF BB Homodimer

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP036 E FN WB 10 µg
Recombinant Human PDGF BB homodimer is an E. coli-derived recombinant protein corresponding to mature full-length human Platelet-Derived Growth Factor BB homodimer (PDGF-BB), a potent mitogen for cells of mesenchymal origin. PDGF is composed of a dimer of two chains, known as the A chain and B chain. The dimer may present as either AA or BB homodimer, or as an AB heterodimer.

Recombinant Human PDGF BB homodimer (PHP036) has an ED50 of 1.0-3.0 ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. After the initial reconstitution step, the product should be diluted to a working concentration using an aqueous buffer containg 0.1% BSA (or any albumin), or diluted in culture media containing serum, such as FCS.
Buffer Solution
30 mM Acetic Acid
Preservative Stabilisers
None present
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution.
Protein Molecular Weight
24.3kD (218 amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P01127 Related reagents
Entrez Gene
PDGFB Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002548 monocyte chemotaxis
GO:0002576 platelet degranulation
GO:0005576 extracellular region
GO:0005161 platelet-derived growth factor receptor binding
GO:0005518 collagen binding
GO:0005788 endoplasmic reticulum lumen
GO:0008083 growth factor activity
GO:0009986 cell surface
GO:0010512 negative regulation of phosphatidylinositol biosynthetic process
GO:0010544 negative regulation of platelet activation
GO:0014911 positive regulation of smooth muscle cell migration
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0042803 protein homodimerization activity
GO:0043406 positive regulation of MAP kinase activity
GO:0043498 cell surface binding
GO:0043536 positive regulation of blood vessel endothelial cell migration
GO:0045740 positive regulation of DNA replication
GO:0046982 protein heterodimerization activity
GO:0048008 platelet-derived growth factor receptor signaling pathway
GO:0048146 positive regulation of fibroblast proliferation
GO:0048407 platelet-derived growth factor binding
GO:0050921 positive regulation of chemotaxis
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of PDGF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.2ng/ml 10ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own systems using appropriate negative/positive controls.
ELISA
PHP036 may be used in ELISA applications with AHP793.
Western Blotting
PHP036 may be used under reducing or non-reducing western blotting conditions with AHP793.