Recombinant Human PAI-1
- Product Type
- Recombinant Protein
|PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis. Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).|
- Target Species
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitute with 100 ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended sotrage, the addition of 0.1% BSA is recommended.
- Purified recombinant PAI-1 expressed in E.coli.
- Buffer Solution
- 10mM Sodium Phosphate
- Preservative Stabilisers
- None present
- Carrier Free
- Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37oC. Half maximal inhibition against 1.0 μg/ml of single chain tPA was obtained at a concentration of 2.0 μg/ml.
- >95% by SDS PAGE and HPLC analysis.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 42.7 kDa (379 amino acid residues).
- Endotoxin Level
- < 1.0 EU/ug
- For research purposes only
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0001300 chronological cell aging
- GO:0002020 protease binding
- GO:0002576 platelet degranulation
- GO:0004252 serine-type endopeptidase activity
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0050829 defense response to Gram-negative bacterium
- GO:0010469 regulation of receptor activity
- View More GO Terms
- GO:0010757 negative regulation of plasminogen activation
- GO:0014912 negative regulation of smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0031012 extracellular matrix
- GO:0031093 platelet alpha granule lumen
- GO:0032757 positive regulation of interleukin-8 production
- GO:0033629 negative regulation of cell adhesion mediated by integrin
- GO:0035491 positive regulation of leukotriene production involved in inflammatory response
- GO:0042730 fibrinolysis
- GO:0043066 negative regulation of apoptosis
- GO:0045766 positive regulation of angiogenesis
- GO:0048260 positive regulation of receptor-mediated endocytosis
- GO:0051918 negative regulation of fibrinolysis
- GO:0061044 negative regulation of vascular wound healing
- GO:0070887 cellular response to chemical stimulus
- GO:0071222 cellular response to lipopolysaccharide
- GO:0090026 positive regulation of monocyte chemotaxis
- GO:2000098 negative regulation of smooth muscle cell-matrix adhesion
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