PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis. Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).
- Target Species
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitute with 100 ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended sotrage, the addition of 0.1% BSA is recommended.
- Purified recombinant PAI-1 expressed in E.coli.
- Buffer Solution
- 10mM Sodium Phosphate
- Preservative Stabilisers
- None present
- Carrier Free
- Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37oC. Half maximal inhibition against 1.0 μg/ml of single chain tPA was obtained at a concentration of 2.0 μg/ml.
- >95% by SDS PAGE and HPLC analysis.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 42.7 kDa (379 amino acid residues).
- Endotoxin Level
- < 1.0 EU/ug
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 3 months from date of reconstitution
- Entrez Gene
- GO Terms
chronological cell aging
serine-type endopeptidase activity
serine-type endopeptidase inhibitor activity
defense response to Gram-negative bacterium
regulation of receptor activity
negative regulation of plasminogen activation
negative regulation of smooth muscle cell migration
platelet alpha granule lumen
positive regulation of interleukin-8 production
negative regulation of cell adhesion mediated by integrin
positive regulation of leukotriene production involved in inflammatory response
negative regulation of apoptosis
positive regulation of angiogenesis
positive regulation of receptor-mediated endocytosis
negative regulation of fibrinolysis
negative regulation of vascular wound healing
cellular response to chemical stimulus
cellular response to lipopolysaccharide
positive regulation of monocyte chemotaxis
negative regulation of smooth muscle cell-matrix adhesion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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