MMP-2
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Recombinant Human MMP-2 antigen produced in E. coli represents human MMP-2 (matrix metalloproteinase 2) containing the entire catalytic N-terminal domain and C-terminal domain. MMP-2 is otherwise known as gelatinase A, a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin.
MMP-2 specifically cleaves gelatin type I, and types IV, V, VII and X collagens, and is also involved in tissue repair, inflammation, tumour invasion, vasculature angiogenesis, and the rupture of atherosclerotic plaques.
Defects in the MMP2 gene are responsible for the autosomal recessive osteolysis disorders known as Torg-Winchester syndrome (Zankl et al. 2007), and multicentric osteolysis nodulosis and arthropathy (Martignetti et al. 2001).
MMP-2 specifically cleaves gelatin type I, and types IV, V, VII and X collagens, and is also involved in tissue repair, inflammation, tumour invasion, vasculature angiogenesis, and the rupture of atherosclerotic plaques.
Defects in the MMP2 gene are responsible for the autosomal recessive osteolysis disorders known as Torg-Winchester syndrome (Zankl et al. 2007), and multicentric osteolysis nodulosis and arthropathy (Martignetti et al. 2001).
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitution
- Reconstitute with 20ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified recombinant MMP-2 expressed in E.coli
- Buffer Solution
Sodium phosphate Calcium chloride - Preservative Stabilisers
- None present
- Activity
- MMP-2 activity was measured by its ability to cleave a chromogenic peptide MMP-2 substrate at room temperature. At an MMP-2 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 25 mins.
- Purity
- >90% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.5 mg/ml after reconstitution
- Endotoxin Level
- < 1.0 EU/ug
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 3 months from date of reconstitution.
More Information
- UniProt
- P08253 Related reagents
- Entrez Gene
- MMP2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0001525 angiogenesis
- GO:0004222 metalloendopeptidase activity
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0030574 collagen catabolic process
- Regulatory
- For research purposes only
Applications of MMP-2
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Functional Assays |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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