Recombinant Human Interleukin-6 protein produced in E. coli constitutes full length non-glycosylated human IL-6 and retainf full bioogical activity. Interleukin-6 is a potent lymphoid cell growth factor, acting on B cells, T cells and hybridoma cells.This preparation has an ED50 of 0.1ng/ml when assayed by proliferation of murine 7TD1 cells.
- Target Species
- Product Form
- Purified recombinant protein - lyophilised
Pack Size: 5 µg
Reconstitute with 50ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Pack Size: 20 µg
Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
- Recombinant protein expressed in E. coli.
- Preservative Stabilisers
- None present.
- >1 x 107 units/mg
- >98% by SDS PAGE analysis and HPLC analysis
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 20.9 kDa (184 amino acids)
- Endotoxin Level
- <0.1 ng/ug
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
- Entrez Gene
- GO Terms
hepatic immune response
neutrophil mediated immunity
positive regulation of acute inflammatory response
positive regulation of leukocyte chemotaxis
interleukin-6 receptor binding
interleukin-6 receptor complex
humoral immune response
growth factor activity
defense response to Gram-positive bacterium
defense response to Gram-negative bacterium
negative regulation of cell proliferation
regulation of vascular endothelial growth factor production
negative regulation of lipid storage
positive regulation of translation
endocrine pancreas development
neuron projection development
response to peptidoglycan
positive regulation of chemokine production
positive regulation of interleukin-6 production
negative regulation of collagen biosynthetic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of apoptosis
positive regulation of gene-specific transcription
positive regulation of MAPKKK cascade
negative regulation of chemokine biosynthetic process
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
regulation of angiogenesis
positive regulation of anti-apoptosis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of B cell activation
positive regulation of immunoglobulin secretion
positive regulation of transcription factor activity
response to glucocorticoid stimulus
defense response to virus
interleukin-6-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Recombinant Human IL-6 (PHP045) may be used as a standard for ELISA applications with biotinylated Rabbit anti Human Interleukin-6 (AHP1040B) for detection.
- Western Blotting
- Recombinant Human IL-6 may be used as the positive control for western blotting applications with Rabbit anti Human Interleukin-6 (AHP1040) for detection.
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Useful Reagents Available
Product Specific References
References for IL-6
Ban, S.A. et al. (2014) Combined immunodeficiency evolving into predominant CD4+ lymphopenia caused by somatic chimerism in JAK3.
J Clin Immunol. 34 (8): 941-53.