IL-6

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Recombinant Human Interleukin-6

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP045A E FN WB 5 µg
PHP045 E FN WB 20 µg
Recombinant Human Interleukin-6 protein produced in E. coli constitutes full length non-glycosylated human IL-6 and retainf full bioogical activity. Interleukin-6 is a potent lymphoid cell growth factor, acting on B cells, T cells and hybridoma cells.This preparation has an ED50 of 0.1ng/ml when assayed by proliferation of murine 7TD1 cells.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Pack Size: 5 µg
Reconstitute with 50ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Pack Size: 20 µg
Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preparation
Recombinant protein expressed in E. coli.
Preservative Stabilisers
None present.
Activity
>1 x 107 units/mg
Purity
>98% by SDS PAGE analysis and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
20.9 kDa (184 amino acids)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Pack Size: 5 µg
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 20 µg
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P05231 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0001781 neutrophil apoptosis
GO:0002384 hepatic immune response
GO:0002446 neutrophil mediated immunity
GO:0002548 monocyte chemotaxis
GO:0002675 positive regulation of acute inflammatory response
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0006959 humoral immune response
GO:0008083 growth factor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0050829 defense response to Gram-negative bacterium
GO:0008285 negative regulation of cell proliferation
GO:0010574 regulation of vascular endothelial growth factor production
GO:0010888 negative regulation of lipid storage
GO:0045727 positive regulation of translation
GO:0030168 platelet activation
GO:0031018 endocrine pancreas development
GO:0031175 neuron projection development
GO:0032494 response to peptidoglycan
GO:0032722 positive regulation of chemokine production
GO:0032755 positive regulation of interleukin-6 production
GO:0032966 negative regulation of collagen biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042102 positive regulation of T cell proliferation
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0043410 positive regulation of MAPKKK cascade
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045765 regulation of angiogenesis
GO:0045768 positive regulation of anti-apoptosis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050871 positive regulation of B cell activation
GO:0051024 positive regulation of immunoglobulin secretion
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0051607 defense response to virus
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of IL-6

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.1 10ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant Human IL-6 (PHP045) may be used as a standard for ELISA applications with biotinylated Rabbit anti Human Interleukin-6 (AHP1040B) for detection.
Western Blotting
Recombinant Human IL-6 may be used as the positive control for western blotting applications with Rabbit anti Human Interleukin-6 (AHP1040) for detection.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human Interleukin-6 AHP1040 0.1 mg E FN* IF P* WB
Rabbit anti Human Interleukin-6:Biotin AHP1040B 50 µg E WB

Product Specific References

References for IL-6

  1. Ban, S.A. et al. (2014) Combined immunodeficiency evolving into predominant CD4+ lymphopenia caused by somatic chimerism in JAK3.
    J Clin Immunol. 34 (8): 941-53.