IL-28A

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Recombinant Human INTERLEUKIN-28A

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP298
Datasheet
E
SDS
10 µg loader

Recominant Human interleukin-28A

Interleukin-28A (IL-28A) also known as interferon lambda-2 (IFNL2) is a member of the IL-10 interferon cytokine family. Similar to interferons, IL-28a is induced by viral infection and has anti-viral activity (Kempuraj et al. 2004). IL-28A can be produced by virus infected cells in the skin, leading to upregulation of proteins that inhibit virus replication. In this way, IL-28A may be involved with clearance of viruses in the skin (Wolk et al. 2010). Studies have also indicated that IL-28A is able to induce the innate and adaptive immune responses to engage in anti-tumor activity (Numasaki et al. 2007).

The recombinant Human interleukin-28A can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-28A antibody, clone G04-5A2 (MCA6210GA) as a capture antibody and biotinylated Mouse anti Human interleukin-28A antibody, clone G02-3A6 (MCA6211B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Recombinant protein expressed in E. coli and purified by ion exchange chromatography
Buffer Solution
50 mM Tris-HCl, pH 8.0, 0.15 M Sodium Chloride
Preservative Stabilisers
1.0% Trehalose
Purity
≥95 % determined by coomassie blue staining of a reducing SDS-PAGE gel
Approx. Protein Concentrations
100 μg/ml after reconstitution
Protein Molecular Weight
21 kDa

Storage Information

Storage
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
Q8IZJ0
Entrez Gene
IL28A
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of IL-28A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human INTERLEUKIN-28A MCA6210GA E 0.1 mg loader
Mouse anti Human INTERLEUKIN-28A:Biotin MCA6211B E 0.1 mg loader

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