IL-28A
Recombinant Human interleukin-28A (PHP298) was analyzed under reducing denaturing conditions (Lane 2). The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand of the image. Bands were visualized using Coomassie stains.
Recombinant Human INTERLEUKIN-28A
- Product Type
- Recombinant Protein
- Specificity
- IL-28A
Antibody Quality and Performance Guarantee
| Recominant Human interleukin-28A Interleukin-28A (IL-28A) also known as interferon lambda-2 (IFNL2) is a member of the IL-10 interferon cytokine family. Similar to interferons, IL-28a is induced by viral infection and has anti-viral activity (Kempuraj et al. 2004). IL-28A can be produced by virus infected cells in the skin, leading to upregulation of proteins that inhibit virus replication. In this way, IL-28A may be involved with clearance of viruses in the skin (Wolk et al. 2010). IL-28A is able to induce the innate and adaptive immune responses to engage in anti-tumor activity (Numasaki et al. 2007). The recombinant Human interleukin-28A can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-28A antibody, clone G04-5A2 (MCA6210GA) as a capture antibody and biotinylated Mouse anti Human interleukin-28A antibody, clone G02-3A6 (MCA6211B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex. - Preparation
- Recombinant protein expressed in E. coli and purified by ion exchange chromatography
- Source
- E.coli
- Buffer Solution
- 50 mM Tris-HCl, pH 8.0, 0.15 M Sodium Chloride
- Preservative Stabilisers
- 1.0% Trehalose
- Purity
- ≥95 % determined by coomassie blue staining of a reducing SDS-PAGE gel
- Approx. Protein Concentrations
- 100 μg/ml after reconstitution
- Protein Molecular Weight
- 21 kDa
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human INTERLEUKIN-28A | MCA6210GA | E | 0.1 mg |
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| Description | Mouse anti Human INTERLEUKIN-28A | ||||||
| Mouse anti Human INTERLEUKIN-28A:Biotin | MCA6211B | E | 0.1 mg |
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| Description | Mouse anti Human INTERLEUKIN-28A:Biotin | ||||||
- Synonyms
- IFN lambda-2
- UniProt
- Q8IZJ0
- Entrez Gene
- IL28A
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
PHP298
100005640
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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