Human IgM | AbD18705_hIgM

Recombinant Human IgM Kappa

Product Type
Recombinant Protein
Clone
AbD18705_hIgM
Isotype
IgM
Specificity
Human IgM
Region
KAPPA

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HCA191
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Recombinant human IgM kappa, clone AbD18705_hIgM is a recombinant human IgM antibody with a kappa light chain.

Clone AbD18705_hIgM is specific for green fluorescent protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a control reagent when using other human antibodies of the same isotype.

Clone AbD18705_hIgM forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.

Product Form
Human IgM antibody selected from the HuCAL phage display library and expressed in a human cell line. This product is supplied as a liquid.
Preparation
Purified IgM prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Green fluorescent protein (GFP)
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

UniProt
P01871
P01834
Entrez Gene
IGKC
IGHM
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0016021 integral to membrane
GO:0005624 membrane fraction
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"