Human IgM | AbD18705_hIgM

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Recombinant Human IgM Kappa

Recombinant Human IgM Kappa

Product Type
Recombinant Protein
Clone
AbD18705_hIgM
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
HCA191
Datasheet
E
SDS
0.1 mg loader

HCA191S
Datasheet
E
SDS
2 ml loader

Recombinant human IgM kappa, clone AbD18705_hIgM is a recombinant human IgM antibody with a kappa light chain.

Clone AbD18705_hIgM is specific for green fluorescent protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a control reagent when using other human antibodies of the same isotype.

Clone AbD18705_hIgM forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.

Product Details

Product Form
Human IgM antibody selected from the HuCAL® phage display library and expressed in a human cell line. This product is supplied as a liquid.
Product Form
Human IgM antibody selected from the HuCAL® phage display library and expressed in a human cell line. This product is supplied as tissue culture supernatant containing 1% FCS in a liquid format.
Preparation
Purified IgM prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
None present
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein (GFP)
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01871
P01834
Entrez Gene
IGKC
IGHM
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0016021 integral to membrane
GO:0005624 membrane fraction
Acknowledgements
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgM

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Recombinant Human IgM Lambda HCA170S E 2 ml loader