Human IgG3 | AbD18705_hIgG3

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Recombinant Human IgG3 Kappa

Product Type
Recombinant Protein
Clone
AbD18705_hIgG3
Isotype
IgG3 Kappa
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA194 E 0.1 mg
Recombinant Human IgG3 kappa, clone AbD18705_hIgG3 is a recombinant human IgG3 antibody with a kappa light chain and a heavy chain belonging to the G3m(b1) allotype, previously designated G3m5. Clone AbD18705_hIgG3 is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG3 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

Clone AbD18705_hIgG3 forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.

Product Details

Product Form
Human IgG3 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green Fluorescent Protein (GFP).
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P01834 Related reagents
P01860 Related reagents
Entrez Gene
IGKC Related reagents
IGHG3 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgG3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human IgG3 Lambda HCA178 0.1 mg E