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IGF-I

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Recombinant Human Insulin-Like Growth Factor I

Product Type
Recombinant Protein
Specificity
IGF-I

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IGF-I (Insulin-like Growth Factor I), is a secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver, which effects a wide range of cells and plays a key role in cell proliferation and growth, inhibition of apoptosis, and is linked with tumour cell growth.

IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. IGF-I production is stimulated by growth hormone (GH). and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant IGF-I expressed in E.coli
Source
E.coli
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
The ED50 was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
Purity
>98% by SDS PAGE/HPLC
Approx. Protein Concentrations
0.1 mg/ml
Protein Molecular Weight
7.6 kDa
Endotoxin Level
< 1.0 EU/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IGF-I

  1. Zhang X et al. (2015) Wnt signaling regulates the stemness of lung cancer stem cells and its inhibitors exert anticancer effect on lung cancer SPC-A1 cells.
    Med Oncol. 32 (4): 462.

Synonyms
Insulin-Like Growth Factor I
UniProt
P05019
Entrez Gene
IGF1
GO Terms
GO:0006260 DNA replication
GO:0002576 platelet degranulation
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0007265 Ras protein signal transduction
GO:0007517 muscle organ development
GO:0008083 growth factor activity
GO:0009441 glycolate metabolic process
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration
GO:0014896 muscle hypertrophy
GO:0014904 myotube cell development
GO:0014911 positive regulation of smooth muscle cell migration
GO:0046326 positive regulation of glucose import
GO:0016942 insulin-like growth factor binding protein complex
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0034392 negative regulation of smooth muscle cell apoptosis
GO:0035630 bone mineralization involved in bone maturation
GO:0040014 regulation of multicellular organism growth
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
GO:0045445 myoblast differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0045740 positive regulation of DNA replication
GO:0045821 positive regulation of glycolysis
GO:0045840 positive regulation of mitosis
GO:0046579 positive regulation of Ras protein signal transduction
GO:0048146 positive regulation of fibroblast proliferation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050679 positive regulation of epithelial cell proliferation
GO:0051450 myoblast proliferation

PHP177

159893 164857 170601

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