IGF-I

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Recombinant Human Insulin-Like Growth Factor I

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP177 Datasheet	FN SDS	0.1 mg	Log in		Get Quote

IGF-I (Insulin-like Growth Factor I), is a secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver, which effects a wide range of cells and plays a key role in cell proliferation and growth, inhibition of apoptosis, and is linked with tumour cell growth.

IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. IGF-I production is stimulated by growth hormone (GH). and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified recombinant IGF-I expressed in E.coli
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: The ED₅₀ was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 10⁵ units/mg.
Purity: >98% by SDS PAGE/HPLC
Approx. Protein Concentrations: 0.1 mg/ml
Protein Molecular Weight: 7.6 kDa
Endotoxin Level: < 1.0 EU/ug

Storage Information

Storage: Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P05019
Entrez Gene: IGF1
GO Terms: GO:0006260 DNA replication; GO:0002576 platelet degranulation; GO:0005158 insulin receptor binding; GO:0005159 insulin-like growth factor receptor binding; GO:0005179 hormone activity; GO:0006916 anti-apoptosis; GO:0006928 cellular component movement; GO:0007265 Ras protein signal transduction; GO:0007517 muscle organ development; GO:0008083 growth factor activity; GO:0009441 glycolate metabolic process; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade; GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration; GO:0014896 muscle hypertrophy; GO:0014904 myotube cell development; GO:0014911 positive regulation of smooth muscle cell migration; GO:0046326 positive regulation of glucose import; GO:0016942 insulin-like growth factor binding protein complex; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030168 platelet activation; GO:0031093 platelet alpha granule lumen; GO:0034392 negative regulation of smooth muscle cell apoptosis; GO:0035630 bone mineralization involved in bone maturation; GO:0040014 regulation of multicellular organism growth; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway; GO:0045445 myoblast differentiation; GO:0045669 positive regulation of osteoblast differentiation; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0045740 positive regulation of DNA replication; GO:0045821 positive regulation of glycolysis; GO:0045840 positive regulation of mitosis; GO:0046579 positive regulation of Ras protein signal transduction; GO:0048146 positive regulation of fibroblast proliferation; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0050679 positive regulation of epithelial cell proliferation; GO:0051450 myoblast proliferation
Regulatory: For research purposes only

Applications of IGF-I

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IGF-I

Zhang X et al. (2015) Wnt signaling regulates the stemness of lung cancer stem cells and its inhibitors exert anticancer effect on lung cancer SPC-A1 cells.
Med Oncol. 32 (4): 462.

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