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Human IgE | AbD00264_hIgE

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Recombinant Human IgE Lambda, clone AbD00264_hIgE thumbnail image 1 Recombinant Human IgE Lambda, clone AbD00264_hIgE thumbnail image 2 Recombinant Human IgE Lambda, clone AbD00264_hIgE thumbnail image 3
Recombinant Human IgE Lambda, clone AbD00264_hIgE gallery image 1
Recombinant Human IgE (HCA171) used as an antigen in an indirect ELISA followed by Mouse anti Human IgE (0100-0413) and horseradish peroxidase conjugated Rabbit F(ab)′2 anti Mouse IgG antibody (STAR13B) as detection reagents
Recombinant Human IgE Lambda, clone AbD00264_hIgE gallery image 2
SDS-PAGE analysis of recombinant Human IgE lambda (HCA171). The sample was analyzed under reducing, denaturing conditions (Lane 2) and non-reducing, denaturing conditions (Lane 3) and visualized using Coomassie staining. The heavy (HC) and light chains (LC) are indicated, respectively
Recombinant Human IgE Lambda, clone AbD00264_hIgE gallery image 3
Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody (0100-0413) as a capture reagent and biotinylated Goat anti Human IgE antibody (STAR147B) as a detection reagent with recombinant human IgE (HCA171) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma nd serum samples diluted 1:5 are indicated in green and orange respectively

Recombinant Human IgE Lambda

Product Type
Recombinant Protein
Clone
AbD00264_hIgE
Isotype
IgE Lambda
Product Code Applications Pack Size List Price Quantity
HCA171
Datasheet
E
SDS
0.1 mg loader

HCA171G
Datasheet
E
SDS
1 mg loader

Recombinant Human IgE lambda, clone AbD00264_hIgE contains a lambda light chain.

Clone AbD00264_hIgE is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.

Product Details

Product Form
Human IgE antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgE prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgE

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
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