FGF Basic
Recombinant Human FGF Basic (PHP105) used to supplement tissue cultuure medium supporting growth of human and canine chondrocyte like cells (CLCs) in vitro.
Image caption:
Indian and Sonic hedgehog facilitate calcification in human and canine chondrocyte‐like cells in vitro. (A) Hedgehog and PTHrP signaling‐related mRNA expression in canine CLC monolayers treated with hypertrophic induction medium supplemented with/without 1 μg/mL IHH or SHH for 7 days. n = 4. **P <.01. ALP, alkaline phosphatase; IHH, Indian hedgehog; PTHR1, PTHrP receptor 1, RUNX2, Runt‐related transcription factor 2; SHH, Sonic hedgehog. Alizarin Red S staining on one representative canine (B) and human (C) CLC donor. CLC monolayers were treated with hypertrophic induction medium supplemented with/without 1 μg/mL IHH or SHH for 7 days. n = 4 per species
From: Bach FC, de Rooij KM, Riemers FM, Snuggs JW, de Jong WAM, Zhang Y, Creemers LB, Chan D, Le Maitre C, Tryfonidou MA.
Hedgehog proteins and parathyroid hormone-related protein are involved in intervertebral disc maturation, degeneration, and calcification.
JOR Spine. 2019 Nov 19;2(4):e1071.
doi: 10.1002/jsp2.1071.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Human FGF basic represents the C-terminal protion of human fibroblast growth factor 2 (A135 - S288).
Fibroblast growth factor basic (FGF basic), also known as FGF 2, is a heparin binding growth factor which has stimulatory activity on a range of cells of mesenchymal, neuroectodermal and endothelial origin.
Note: FGF basic is sensitive to acidic conditions.
Fibroblast growth factor basic (FGF basic), also known as FGF 2, is a heparin binding growth factor which has stimulatory activity on a range of cells of mesenchymal, neuroectodermal and endothelial origin.
Note: FGF basic is sensitive to acidic conditions.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein expressed in E. coli - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml Tris (5mM, pH7.6).
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Further dilutions may be prepared in a buffer containing a carrier protein (eg 0.1% BSA). - Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- 2 x 106 units/mg
- Purity
- >95% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml after reconstitution.
- Protein Molecular Weight
- 17.2 kD (154 amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
Applications of FGF Basic
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4 ng/well | |
| Functional Assays | 0.1 | 10 ng/ml | |
| Western Blotting | 1.5 | 3.0 ng/lane |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for FGF Basic
-
Svendsen, C.N. et al. (1997) Long-term survival of human central nervous system progenitor cells transplanted into a rat model of Parkinson's disease.
Exp Neurol. 148: 135-46. -
Kim, T.H. et al. (2005) Recombinant human prothrombin kringle-2 induces bovine capillary endothelial cell cycle arrest at G0-G1 phase through inhibition of cyclin D1/CDK4 complex: modulation of reactive oxygen species generation and up-regulation of cyclin-dependent kinase inhibitors.
Angiogenesis. 8: 307-14. -
van Beuningen, HM et al. (2014) Inhibition of TAK1 and/or JAK can rescue impaired chondrogenic differentiation of human mesenchymal stem cells in osteoarthritis-like conditions.
Tissue Eng Part A. 20 (15-16): 2243-52. -
Pleumeekers, M.M. et al. (2014) The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage.
Eur Cell Mater. 27: 264-80. -
Willems, N. et al. (2015) Intradiscal application of rhBMP-7 does not induce regeneration in a canine model of spontaneous intervertebral disc degeneration.
Arthritis Res Ther. 17: 137. -
Pleumeekers, M.M. et al. (2015) Cartilage regeneration in the head and neck area: Combination of ear or nasal chondrocytes and mesenchymal stem cells improves cartilage production: Cell combinations for cartilage production.
Plast Reconstr Surg. Aug 10. [Epub ahead of print] -
Dimitrellos, V. et al. (2003) Capillary electrophoresis and enzyme solid phase assay for examining the purity of a synthetic heparin proteoglycan-like conjugate and identifying binding to basic fibroblast growth factor.
Biomed Chromatogr. 17 (1): 42-7. -
Narcisi R et al. (2015) Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation.
Stem Cell Reports. 4 (3): 459-72. -
Lolli, A. et al. (2016) Silencing of Antichondrogenic MicroRNA-221 in Human Mesenchymal Stem Cells Promotes Cartilage Repair In Vivo..
Stem Cells. 34 (7): 1801-11. -
de Kroon, L. M. G. et al. (2016) Activin and Nodal Are Not Suitable Alternatives to TGF for Chondrogenic Differentiation of Mesenchymal Stem Cells
Cartilage. Sep 7 [Epub ahead of print] -
Cleary, M.A. et al. (2016) Expression of CD105 on expanded mesenchymal stem cells does not predict their chondrogenic potential.
Osteoarthritis Cartilage. 24 (5): 868-72. -
Grotenhuis, N. et al. (2016) Biomaterials Influence Macrophage-Mesenchymal Stem Cell Interaction In Vitro.
Tissue Eng Part A. 22 (17-18): 1098-107. -
Rodrigues, A.I. et al. (2017) Calcium phosphates and silicon: exploring methods of incorporation.
Biomater Res. 21: 6. -
Le, B.Q. et al. (2015) High-Throughput Screening Assay for the Identification of Compounds Enhancing Collagenous Extracellular Matrix Production by ATDC5 Cells.
Tissue Eng Part C Methods. 21 (7): 726-36. -
Le, B.Q. et al. (2017) An Approach to In Vitro Manufacturing of Hypertrophic Cartilage Matrix for Bone Repair.
Bioengineering (Basel). 4 (2)Apr 20 [Epub ahead of print]. -
Bach, F.C. et al. (2017) Link-N: The missing link towards intervertebral disc repair is species-specific.
PLoS One. 12 (11): e0187831. -
Pleumeekers, M.M. et al. (2018) Trophic effects of adipose-tissue-derived and bone-marrow-derived mesenchymal stem cells enhance cartilage generation by chondrocytes in co-culture.
PLoS One. 13 (2): e0190744. -
Narcisi, R. et al. (2021) Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.
Methods Mol Biol. 2221: 15-28. -
Tellegen, A. et al. (2021) Intra-Articular Slow-Release Triamcinolone Acetonide from Polyesteramide Microspheres as a Treatment for Osteoarthritis
Pharmaceutics. 13 (3): 372. -
Bach, F.C. et al. (2019) Hedgehog proteins and parathyroid hormone-related protein are involved in intervertebral disc maturation, degeneration, and calcification.
JOR Spine. 2 (4): e1071. -
Lolli, A. et al. (2019) Hydrogel-based delivery of antimiR-221 enhances cartilage regeneration by endogenous cells.
J Control Release. 309: 220-30. -
Vainieri, M.L. et al. (2020) Evaluation of biomimetic hyaluronic-based hydrogels with enhanced endogenous cell recruitment and cartilage matrix formation.
Acta Biomater. 101: 293-303. -
Khatab, S. et al. (2020) MSC encapsulation in alginate microcapsules prolongs survival after intra-articular injection, a longitudinal in vivo cell and bead integrity tracking study.
Cell Biol Toxicol. 36 (6): 553-570. -
Teunissen, M. et al. (2021) The lower in vitro. chondrogenic potential of canine adipose tissue-derived mesenchymal stromal cells (MSC) compared to bone marrow-derived MSC is not improved by BMP-2 or BMP-6.
Vet J. 269: 105605. -
Sivasubramaniyan, K. et al. (2019) Cell-surface markers identify tissue resident multipotential stem/stromal cell subsets in synovial intimal and sub-intimal compartments with distinct chondrogenic properties.
Osteoarthritis Cartilage. 27 (12): 1831-1840.
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