Fibroblast growth factor basic (FGF basic), also known as FGF 2, is a heparin binding growth factor which has stimulatory activity on a range of cells of mesenchymal, neuroectodermal and endothelial origin.
Note: FGF basic is sensitive to acidic conditions.
- Target Species
- Product Form
- Purified recombinant protein expressed in E. coli - lyophilized
- Reconstitute with 0.5 ml Tris (5mM, pH7.6).
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Further dilutions may be prepared in a buffer containing a carrier protein (eg 0.1% BSA).
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- None present
- Carrier Free
- 2 x 106 units/mg
- >95% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml after reconstitution.
- Protein Molecular Weight
- 17.2 kD (154 amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Applications of FGF Basic
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays||0.1||10 ng/ml|
|Western Blotting||1.5||3.0 ng/lane|
Product Specific References
References for FGF Basic
Svendsen, C.N. et al. (1997) Long-term survival of human central nervous system progenitor cells transplanted into a rat model of Parkinson's disease.
Exp Neurol. 148: 135-46.
Kim, T.H. et al. (2005) Recombinant human prothrombin kringle-2 induces bovine capillary endothelial cell cycle arrest at G0-G1 phase through inhibition of cyclin D1/CDK4 complex: modulation of reactive oxygen species generation and up-regulation of cyclin-dependent kinase inhibitors.
Angiogenesis. 8: 307-14.
van Beuningen, HM et al. (2014) Inhibition of TAK1 and/or JAK can rescue impaired chondrogenic differentiation of human mesenchymal stem cells in osteoarthritis-like conditions.
Tissue Eng Part A. 20 (15-16): 2243-52.
Pleumeekers, M.M. et al. (2014) The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage.
Eur Cell Mater. 27: 264-80.
Willems, N. et al. (2015) Intradiscal application of rhBMP-7 does not induce regeneration in a canine model of spontaneous intervertebral disc degeneration.
Arthritis Res Ther. 17: 137.
Pleumeekers, M.M. et al. (2015) Cartilage regeneration in the head and neck area: Combination of ear or nasal chondrocytes and mesenchymal stem cells improves cartilage production: Cell combinations for cartilage production.
Plast Reconstr Surg. Aug 10. [Epub ahead of print]
Dimitrellos, V. et al. (2003) Capillary electrophoresis and enzyme solid phase assay for examining the purity of a synthetic heparin proteoglycan-like conjugate and identifying binding to basic fibroblast growth factor.
Biomed Chromatogr. 17 (1): 42-7.
Narcisi R et al. (2015) Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation.
Stem Cell Reports. 4 (3): 459-72.
Lolli A et al. (2016) Silencing of anti-chondrogenic microRNA-221 in human mesenchymal stem cells promotes cartilage repair in vivo.
Stem Cells. Mar 1. [Epub ahead of print]
de Kroon, L. M. G. et al. (2016) Activin and Nodal Are Not Suitable Alternatives to TGF for Chondrogenic Differentiation of Mesenchymal Stem Cells
Cartilage. Sep 7 [Epub ahead of print]
Cleary, M.A. et al. (2016) Expression of CD105 on expanded mesenchymal stem cells does not predict their chondrogenic potential.
Osteoarthritis Cartilage. 24 (5): 868-72.
Grotenhuis, N. et al. (2016) Biomaterials Influence Macrophage-Mesenchymal Stem Cell Interaction In Vitro.
Tissue Eng Part A. 22 (17-18): 1098-107.
Rodrigues, A.I. et al. (2017) Calcium phosphates and silicon: exploring methods of incorporation.
Biomater Res. 21: 6.
Le, B.Q. et al. (2015) High-Throughput Screening Assay for the Identification of Compounds Enhancing Collagenous Extracellular Matrix Production by ATDC5 Cells.
Tissue Eng Part C Methods. 21 (7): 726-36.
Le, B.Q. et al. (2017) An Approach to In Vitro Manufacturing of Hypertrophic Cartilage Matrix for Bone Repair.
Bioengineering (Basel). 4 (2)Apr 20 [Epub ahead of print].
Bach, F.C. et al. (2017) Link-N: The missing link towards intervertebral disc repair is species-specific.
PLoS One. 12 (11): e0187831.
Pleumeekers, M.M. et al. (2018) Trophic effects of adipose-tissue-derived and bone-marrow-derived mesenchymal stem cells enhance cartilage generation by chondrocytes in co-culture.
PLoS One. 13 (2): e0190744.
Narcisi, R. et al. (2021) Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.
Methods Mol Biol. 2221: 15-28.
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