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Recombinant Human FGF Basic
- Product Type
- Recombinant Protein
- FGF Basic
|Recombinant Human FGF basic represents the C-terminal protion of human fibroblast growth factor 2 (A135 - S288).
Fibroblast growth factor basic (FGF basic), also known as FGF 2, is a heparin binding growth factor which has stimulatory activity on a range of cells of mesenchymal, neuroectodermal and endothelial origin.
Note: FGF basic is sensitive to acidic conditions.
- Target Species
- Product Form
- Purified recombinant protein expressed in E. coli - lyophilized
- Reconstitute with 0.5 ml Tris (5mM, pH7.6).
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Further dilutions may be prepared in a buffer containing a carrier protein (eg 0.1% BSA).
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- None present
- Carrier Free
- 2 x 106 units/mg
- >95% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml after reconstitution.
- Protein Molecular Weight
- 17.2 kD (154 amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
- For research purposes only
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays||0.1||10 ng/ml|
|Western Blotting||1.5||3.0 ng/lane|
References for FGF Basic
Svendsen, C.N. et al. (1997) Long-term survival of human central nervous system progenitor cells transplanted into a rat model of Parkinson's disease.
Exp Neurol. 148: 135-46.
Dimitrellos, V. et al. (2003) Capillary electrophoresis and enzyme solid phase assay for examining the purity of a synthetic heparin proteoglycan-like conjugate and identifying binding to basic fibroblast growth factor.
Biomed Chromatogr. 17 (1): 42-7.
Kim, T.H. et al. (2005) Recombinant human prothrombin kringle-2 induces bovine capillary endothelial cell cycle arrest at G0-G1 phase through inhibition of cyclin D1/CDK4 complex: modulation of reactive oxygen species generation and up-regulation of cyclin-dependent kinase inhibitors.
Angiogenesis. 8: 307-14.
van Beuningen, HM et al. (2014) Inhibition of TAK1 and/or JAK can rescue impaired chondrogenic differentiation of human mesenchymal stem cells in osteoarthritis-like conditions.
Tissue Eng Part A. 20 (15-16): 2243-52.
Pleumeekers, M.M. et al. (2014) The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage.
Eur Cell Mater. 27: 264-80.
Narcisi R et al. (2015) Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation.
Stem Cell Reports. 4 (3): 459-72.
Quang Le, B. et al. (2015) High-Throughput Screening Assay for the Identification of Compounds Enhancing Collagenous Extracellular Matrix Production by ATDC5 Cells.
Tissue Eng Part C Methods. 21 (7): 726-36.
Willems, N. et al. (2015) Intradiscal application of rhBMP-7 does not induce regeneration in a canine model of spontaneous intervertebral disc degeneration.
Arthritis Res Ther. 17: 137.
View The Latest Product References
Pleumeekers, M.M. et al. (2015) Cartilage Regeneration in the Head and Neck Area: Combination of Ear or Nasal Chondrocytes and Mesenchymal Stem Cells Improves Cartilage Production.
Plast Reconstr Surg. 136 (6): 762e-774e.
Lolli, A. et al. (2016) Silencing of Antichondrogenic MicroRNA-221 in Human Mesenchymal Stem Cells Promotes Cartilage Repair In Vivo..
Stem Cells. 34 (7): 1801-11.
Cleary, M.A. et al. (2016) Expression of CD105 on expanded mesenchymal stem cells does not predict their chondrogenic potential.
Osteoarthritis Cartilage. 24 (5): 868-72.
Grotenhuis, N. et al. (2016) Biomaterials Influence Macrophage-Mesenchymal Stem Cell Interaction In Vitro.
Tissue Eng Part A. 22 (17-18): 1098-107.
Kroon, L.M.G. et al. (2017) Activin and Nodal Are Not Suitable Alternatives to TGFβ for Chondrogenic Differentiation of Mesenchymal Stem Cells.
Cartilage. 8 (4): 432-8.
Rodrigues, A.I. et al. (2017) Calcium phosphates and silicon: exploring methods of incorporation.
Biomater Res. 21: 6.
Quang Le, B. et al. (2017) An Approach to In Vitro Manufacturing of Hypertrophic Cartilage Matrix for Bone Repair.
Bioengineering (Basel). 4 (2): 35.
Bach, F.C. et al. (2017) Link-N: The missing link towards intervertebral disc repair is species-specific.
PLoS One. 12 (11): e0187831.
Pleumeekers, M.M. et al. (2018) Trophic effects of adipose-tissue-derived and bone-marrow-derived mesenchymal stem cells enhance cartilage generation by chondrocytes in co-culture.
PLoS One. 13 (2): e0190744.
Bach, F.C. et al. (2019) Hedgehog proteins and parathyroid hormone-related protein are involved in intervertebral disc maturation, degeneration, and calcification.
JOR Spine. 2 (4): e1071.
Lolli, A. et al. (2019) Hydrogel-based delivery of antimiR-221 enhances cartilage regeneration by endogenous cells.
J Control Release. 309: 220-30.
Sivasubramaniyan, K. et al. (2019) Cell-surface markers identify tissue resident multipotential stem/stromal cell subsets in synovial intimal and sub-intimal compartments with distinct chondrogenic properties.
Osteoarthritis Cartilage. 27 (12): 1831-40.
Vainieri, M.L. et al. (2020) Evaluation of biomimetic hyaluronic-based hydrogels with enhanced endogenous cell recruitment and cartilage matrix formation.
Acta Biomater. 101: 293-303.
Khatab, S. et al. (2020) MSC encapsulation in alginate microcapsules prolongs survival after intra-articular injection, a longitudinal in vivo cell and bead integrity tracking study.
Cell Biol Toxicol. 36 (6): 553-570.
Narcisi, R. et al. (2021) Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.
Methods Mol Biol. 2221: 15-28.
Tellegen, A. et al. (2021) Intra-Articular Slow-Release Triamcinolone Acetonide from Polyesteramide Microspheres as a Treatment for Osteoarthritis
Pharmaceutics. 13 (3): 372.
Teunissen, M. et al. (2021) The lower in vitro. chondrogenic potential of canine adipose tissue-derived mesenchymal stromal cells (MSC) compared to bone marrow-derived MSC is not improved by BMP-2 or BMP-6.
Vet J. 269: 105605.
Du, J. et al. (2022) Intradiscal injection of human recombinant BMP-4 does not reverse intervertebral disc degeneration induced by nuclectomy in sheep.
J Orthop Translat. 37: 23-36.
Basatvat, S. et al. (2023) Harmonization and standardization of nucleus pulposus cell extraction and culture methods
JOR SPINE. Jan 10 [Epub ahead of print].
Schwab, A. 41:42-53. (2023) Modulating design parameters to drive cell invasion into hydrogels for osteochondral tissue formation
J Orthop Translat.
Tryfonidou, M. et al. (2023) Notochordal cell-derived matrix inhibits MAPK signaling in the degenerative disc environment
European Cells and Materials. 46: 57-90.
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