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Ferritin

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Native Human Ferritin thumbnail image 1 Native Human Ferritin thumbnail image 2
Native Human Ferritin gallery image 1
Sandwich ELISA analysis of human ferritin using Mouse anti Human ferritin antibody, clone F23 (4420-3010) as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 (4420-3904) as a detection reagent with purified human ferritin (4420-4804) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included undiluted
Native Human Ferritin gallery image 2
Sandwich ELISA analysis of human ferritin using Mouse anti Human ferritin antibody, clone F31 as a capture reagent and biotinylated Mouse anti Human ferritin antibody, clone 321B7 (4420-3904) as a detection reagent with purified human ferritin (4420-4804) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included undiluted

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Native Human Ferritin

Product Type
Purified Protein
Specificity
Ferritin

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Product Code Applications Pack Size List Price Your Price Qty
4420-4804
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
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Native human Ferritin is a purified preparation of human ferritin from liver tissue. Ferritin is a globular protein composed of 24 identical subunits responsible for storing iron in an available non-toxic form. Ferritin is found primarily in hepatic tissue. Serum levels of ferritin are used as an indicator for anaemia and restless leg syndrome. Ferrin levels are a direct correlate for the total amount of irron stored in the body

Target Species
Human
Product Form
Purified protein from human liver - liquid
Preparation
Multiple purification steps
Buffer Solution
TRIS buffered sodium chloride pH 7-8
Preservative Stabilisers
<0.01% bromo-nitro-dioxane
<0.01% methylisothiazolone
Purity
SDS PAGE: >95%
Approx. Protein Concentrations
3.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for Ferritin

  1. Addison, J.M. et al. (1983) The amino acid sequence of human liver apoferritin.
    FEBS Lett. 164 (1): 139-44.
  2. Na, Y. et al. (2019) Carbon nanotube facilitated interface formation for enhanced protein sensing in electrosynthesized molecular imprinting
    ACS Applied Bio Materials. Aug 29 [Epub ahead of print].

UniProt
P02792
P02794
Entrez Gene
FTL
FTH1
GO Terms
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0016491 oxidoreductase activity
GO:0006826 iron ion transport
GO:0006879 cellular iron ion homeostasis
GO:0006892 post-Golgi vesicle-mediated transport
GO:0008043 intracellular ferritin complex
GO:0008199 ferric iron binding
GO:0042802 identical protein binding
GO:0016044 cellular membrane organization
GO:0005515 protein binding
GO:0004322 ferroxidase activity
GO:0006880 intracellular sequestering of iron ion
GO:0006955 immune response
GO:0008283 cell proliferation
GO:0008285 negative regulation of cell proliferation

4420-4804

154058 161169 170726

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