Recombinant Human erythropoietein alpha is recombinant human erythropoietein alpha, produced in CHO cells. It is a single polypeptide chain containing 166 amino acids with a predicted molecular mass of 21,000 Daltons and an apparent molecular mass of 30,400 Daltons.
- Target Species
- Product Form
- Recombinant protein - lyophilised
- Reconstitute with 250ul sterile deionised water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of a carrier protein (0.1% HSA or BSA) is recommended.
- Buffer Solution
- Citrate buffered saline
- Preservative Stabilisers
- None present.
- The specific activity was measured by normocythaemic mice and found to be 150,000 IU/mg
- >98.0% by HPLC analysis and SDS-PAGE
- Approx. Protein Concentrations
- Total protein concentration 0.2mg/ml after reconstitution
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
erythropoietin receptor binding
positive regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of gene-specific transcription
positive regulation of DNA replication
positive regulation of Ras protein signal transduction
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Erythropoietin Alpha
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Product Specific References
References for Erythropoietin Alpha
Taylor, N.D. et al. (2010) Anti-glycophorin single-chain Fv fusion to low-affinity mutant erythropoietin improves red blood cell-lineage specificity.
Protein Eng Des Sel. 23: 251-60.