Erythropoietin Alpha

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Recombinant Human Erythropoietin Alpha

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1538 E FN 50 µg
Recombinant Human erythropoietein alpha is recombinant human erythropoietein alpha, produced in CHO cells. It is a single polypeptide chain containing 166 amino acids with a predicted molecular mass of 21,000 Daltons and an apparent molecular mass of 30,400 Daltons.

Product Details

Target Species
Human
Product Form
Recombinant protein - lyophilised
Reconstitution
Reconstitute with 250ul sterile deionised water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of a carrier protein (0.1% HSA or BSA) is recommended.
Buffer Solution
Citrate buffered saline
Preservative Stabilisers
None present.
Activity
The specific activity was measured by normocythaemic mice and found to be 150,000 IU/mg
Purity
>98.0% by HPLC analysis and SDS-PAGE
Approx. Protein Concentrations
Total protein concentration 0.2mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P01588 Related reagents
Entrez Gene
EPO Related reagents
GO Terms
GO:0007165 signal transduction
GO:0005128 erythropoietin receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0008015 blood circulation
GO:0008284 positive regulation of cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043193 positive regulation of gene-specific transcription
GO:0043249 erythrocyte maturation
GO:0045740 positive regulation of DNA replication
GO:0046579 positive regulation of Ras protein signal transduction
Regulatory
For research purposes only

Applications of Erythropoietin Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Erythropoietin Alpha

  1. Taylor, N.D. et al. (2010) Anti-glycophorin single-chain Fv fusion to low-affinity mutant erythropoietin improves red blood cell-lineage specificity.
    Protein Eng Des Sel. 23: 251-60.