Elastase

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Native Human Elastase (Neutrophil)

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4050-0184 E 0.1 mg
Human Elastase is a 28.5kDa serine protease that has been purified from human neutrophils. Elastase is also present in the pancreas, leucocytes and serum.

Product Details

Target Species
Human
Product Form
Purified protein from human neutrophils - liquid
Buffer Solution
50mM sodium acetate, pH 5.5
150mM sodium chloride
Preservative Stabilisers
None present.
Activity
20-22 U/mg protein

1 unit hydrolyzes one µmole per minute of MeO-Suc-Ala-Ala-Pro-Val-pNA at 25ºC, in 100mM Tris-Hcl, pH 7.5 with 500mM NaCl.
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
1.0 mg/ml
Molecular Weight
28.5 kDa

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P08246 Related reagents
Entrez Gene
ELANE Related reagents
GO Terms
GO:0005576 extracellular region
GO:0030141 stored secretory granule
GO:0006508 proteolysis
GO:0006874 cellular calcium ion homeostasis
GO:0008201 heparin binding
GO:0009411 response to UV
GO:0009986 cell surface
GO:0019955 cytokine binding
GO:0030163 protein catabolic process
GO:0043406 positive regulation of MAP kinase activity
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045415 negative regulation of interleukin-8 biosynthetic process
GO:0045416 positive regulation of interleukin-8 biosynthetic process
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050728 negative regulation of inflammatory response
GO:0050922 negative regulation of chemotaxis
GO:0051635 bacterial cell surface binding
Regulatory
For research purposes only

Applications of Elastase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.