Defensin Beta 2

Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 0.2ml 10 mM acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
- Preparation
- Purified recombinant BD-2, expressed in E. coli
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- Determined by its ability to chemoattract immature dendritic cells using a concentration of 10.0-100.0 ng/ml
- Purity
- >98% by SDS PAGE and HPLC
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 4.3 kDa (41 amino acid residues)
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- O15263
- Entrez Gene
- DEFB4A
- GO Terms
- GO:0042742 defense response to bacterium
- GO:0005576 extracellular region
- GO:0006935 chemotaxis
- GO:0006955 immune response
- GO:0007186 G-protein coupled receptor protein signaling pathway
- Regulatory
- For research purposes only
Applications of Defensin Beta 2
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.2 | 0.4ng/well | |
Functional Assays | 10 | 100ng/ml | |
Western Blotting | 1.5 | 3.0ng/lane |
- ELISA
- Recombinant human BD-2 may be used as a standard in ELISA applications with either a purified human BD-2 antibody (AHP849) or a biotinylated human BD-2 antibody (AHP489B).
- Western Blotting
- Recombinant human BD-2 may be used as the positive control in Western Blotting applications with either a purified human BD-2 antibody (AHP849) or a biotinylated human BD-2 antibody (AHP489B).
Product Specific References
References for Defensin Beta 2
-
Bals, R. et al. (1998) Human beta-Defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung.
J. Clin. Invest. 102: 874-880. -
Yang, D. et al. (1999) Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6.
Science 286: 525-528.
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