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Defensin Beta 2

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Recombinant Human Defensin Beta 2

Product Type
Recombinant Protein
Specificity
Defensin Beta 2

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Recombinant human defensin beta-2 (BD-2), is a 4.3 kDa peptide comprised of 41 amino acid residues. BD-2, a member of the beta defensin family, is secreted at epithelial surfaces of the skin and respiratory tract and by some leukocytes. This defensin is induced by bacterial products and cytokines during inflammation and functions as part of the innate immune system, having a wide ranging antimicrobial activity. BD-2 also functions as a chemoattractant to immature dendritic cells and memory T cells and acts as a ligand for TLR4, upregulating co-stimulatory molecules and inducing dendritic cell maturation.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.2ml 10 mM acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation
Purified recombinant BD-2, expressed in E. coli
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
Determined by its ability to chemoattract immature dendritic cells using a concentration of 10.0-100.0 ng/ml
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
4.3 kDa (41 amino acid residues)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 10 100ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human BD-2 may be used as a standard in ELISA applications with either a purified human BD-2 antibody (AHP849) or a biotinylated human BD-2 antibody (AHP489B).
Western Blotting
Recombinant human BD-2 may be used as the positive control in Western Blotting applications with either a purified human BD-2 antibody (AHP849) or a biotinylated human BD-2 antibody (AHP489B).

References for Defensin Beta 2

  1. Bals, R. et al. (1998) Human beta-Defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung.
    J. Clin. Invest. 102: 874-880.
  2. Yang, D. et al. (1999) Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6.
    Science 286: 525-528.

Synonyms
BD-2
UniProt
O15263
Entrez Gene
DEFB4A
GO Terms
GO:0042742 defense response to bacterium
GO:0005576 extracellular region
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0007186 G-protein coupled receptor protein signaling pathway
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