Defensin Alpha 1

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Recombinant Human Defensin Alpha 1

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP208 E FN WB 20 µg
Recombinant Human Defensin-α-1 is a 3.4kDa peptide comprised of 30 amino acid residues corresponding to full-length mature human defenstin alpha 1. Defensin-α-1, otherwise known as HNP-1, is a secreted cationic antimicrobial peptide and member of the alpha-defensin family, which plays an important role in the innate immune response against microbial infections.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant defensin-α-1 expressed in E.coli
Preservative Stabilisers
None present
Activity
Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0-10.0 ng/ml.
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Molecular Weight
3.4kD (30 amino acid residues)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P59665 Related reagents
Entrez Gene
DEFA1 Related reagents
GO Terms
GO:0042742 defense response to bacterium
GO:0050832 defense response to fungus
GO:0009615 response to virus
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0031640 killing of cells of other organism
Regulatory
For research purposes only

Applications of Defensin Alpha 1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human defensin-α-1 may be used as a standard for ELISA applications with either a purified anti human defensin-α-1 antibody (AHP1781) or a biotinylated anti human defensin-α-1 antibody (AHP1781B).
Western Blotting
Recombinant human defensin-α-1 may be used as a positive control for Western Blotting applications with either a purifed human defensin-α-1 antibody (AHP1781) or a biotinylated human defensin-α-1 antibody (AHP1781B).