Defensin Alpha 1

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Recombinant Human Defensin Alpha 1

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP208 Datasheet	E FN WB SDS	20 µg	Log in		Get Quote

Summary
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Recombinant Human Defensin-α-1 is a 3.4kDa peptide comprised of 30 amino acid residues corresponding to full-length mature human defenstin alpha 1. Defensin-α-1, otherwise known as HNP-1, is a secreted cationic antimicrobial peptide and member of the alpha-defensin family, which plays an important role in the innate immune response against microbial infections.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 0.2 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified recombinant defensin-α-1 expressed in E.coli
Preservative Stabilisers: None present
Carrier Free: Yes
Activity: Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0 - 10.0 ng/ml
Purity: >98% by SDS PAGE and HPLC
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution
Protein Molecular Weight: 3.4 kDa (30 amino acid residues)
Endotoxin Level: < 1.0 EU/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 3 months from date of reconstitution

More Information

UniProt: P59665
Entrez Gene: DEFA1
GO Terms: GO:0042742 defense response to bacterium; GO:0050832 defense response to fungus; GO:0009615 response to virus; GO:0005615 extracellular space; GO:0006935 chemotaxis; GO:0006955 immune response; GO:0031640 killing of cells of other organism
Regulatory: For research purposes only

Applications of Defensin Alpha 1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays
Western Blotting	1.5	3.0ng/lane

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: Recombinant human defensin-α-1 may be used as a standard for ELISA applications with a purified anti human defensin-α-1 antibody (AHP1781).
Western Blotting: Recombinant human defensin-α-1 may be used as a positive control for Western Blotting applications with a purifed human defensin-α-1 antibody (AHP1781).

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