CTGF

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Recombinant Human CTGF

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP174 E FN WB 20 µg
CTGF (connective tissue growth factor) is a heparin-binding pleiotropic growth factor and member of the CCN family of secreted cysteine rich regulatory proteins, which interacts with other growth factors including transforming growth factor beta (TGF-beta), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMPs).

CTGF promotes the adhesion of epithelial cells and fibroblasts, stimulates integrin expression, and promotes endothelial cell survival, migration and adhesion, implicating CTGF in angiogenesis. CTGF is involved in chondrogenesis and potentially skeletogenesis, induces extracellular matrix formation and has also been implicated in wound healing.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.2ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant CTGF expressed in E.coli
Buffer Solution
10mM Sodium Acetate
Preservative Stabilisers
None present
Activity
Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 µg/ml.
Purity
>95% by SDS PAGE/HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution
Molecular Weight
11.2kD (98 amino acid residues)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P29279 Related reagents
Entrez Gene
CTGF Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0006260 DNA replication
GO:0001558 regulation of cell growth
GO:0005520 insulin-like growth factor binding
GO:0005578 proteinaceous extracellular matrix
GO:0008201 heparin binding
GO:0008544 epidermis development
GO:0009611 response to wounding
GO:0044255 cellular lipid metabolic process
Regulatory
For research purposes only

Applications of CTGF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1.0 2.0ug/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PHP174 may be used as a standard in a sandwich ELISA with AHP1278 as a capture reagent and AHP1278B as the detection reagent.
Western Blotting
PHP174 may be used as a positive control for western blotting for either AHP1278 or AHP1278B.

Product Specific References

References for CTGF

  1. Waddell, J.M. et al. (2011) CTGF expression is up-regulated by PROK1 in early pregnancy and influences HTR-8/Svneo cell adhesion and network formation.
    Hum Reprod. 26 (1): 67-75.