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Collagen I

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Sandwich ELISA analysis of human ferritin using Mouse anti Human collagen I/II/III antibody, clone NLI/22 as a capture reagent and biotinylated Mouse anti Human collagen I antibody as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4.

Native Human Collagen I

Product Type
Purified Protein
Specificity
Collagen I

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Product Code Applications Pack Size List Price Your Price Qty
2150-0030
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.

Target Species
Human
Product Form
Purified protein from human placenta - lyophilized
Reconstitution
Use 0.05 - 0.5 M acetic acid, pH 2.5 at 4ºC.
Preparation
Collagen from washed dissected tissue was dissolved in acetic acid after mild pepsin treatment. Collagen type I was then purified using differential salt precipitation.
Preservative Stabilisers
None present
Purity
>90%
Protein Molecular Weight
300 kDa
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
The human source was tested and found negative for HBsAg ans HIV-1/2 antibodies.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt
P02452
P08123
Entrez Gene
COL1A1
COL1A2

2150-0030

151746 157150 160182 165124

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