Collagen I

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Native Human Collagen I

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0030 E 0.1 mg
Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.

Product Details

Target Species
Human
Product Form
Purified protein from human placenta - lyophilised
Reconstitution
Use 0.05-0.5 M acetic acid, pH 2.5 at 4ÂșC.
Preparation
Collagen from washed dissected tissue was dissolved in acetic acid after mild pepsin treatment. Collagen type I was then purified using differential salt precipitation.
Preservative Stabilisers
None present.
Purity
>90%
Molecular Weight
300 kDa

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02452 Related reagents
P08123 Related reagents
Entrez Gene
COL1A1 Related reagents
COL1A2 Related reagents
Acknowledgements
The human source was tested and found negative for HBsAg ans HIV-1/2 antibodies.
Regulatory
For research purposes only

Applications of Collagen I

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.